Extending Bio-SAXS measurements of Single-Stranded DNA-Binding Proteins: Radiation Protection of G5P by Cosolutes
- Small-angle X-ray scattering (SAXS) can be used for structural de- termination of biological macromolecules and polymers in their na- tive states. To improve the reliability of such experiments, the re- duction of radiation damage occurring from exposure to X-rays is needed.One method, is the use of scavenger molecules that protect macromolecules against radicals produced by radiation exposure.In this study we investigate the feasibility to apply the compatible solute, osmolyte and radiation protector Ectoine (THP(B)) as a scavenger throughout SAXS measurements of single-stranded DNA-binding protein Gene-V Protein (G5P/GVP). Therefore we monitor the radiation induced changes of G5P during bio-SAXS. The resulting microscopic energy-damage relation was determined by particle scattering simu- lations with TOPAS/Geant4. The results are interpreted in terms of radical scavenging as well as post-irradiation effects, related to preferential-exclusion from the protein surface. Thus, EctoineSmall-angle X-ray scattering (SAXS) can be used for structural de- termination of biological macromolecules and polymers in their na- tive states. To improve the reliability of such experiments, the re- duction of radiation damage occurring from exposure to X-rays is needed.One method, is the use of scavenger molecules that protect macromolecules against radicals produced by radiation exposure.In this study we investigate the feasibility to apply the compatible solute, osmolyte and radiation protector Ectoine (THP(B)) as a scavenger throughout SAXS measurements of single-stranded DNA-binding protein Gene-V Protein (G5P/GVP). Therefore we monitor the radiation induced changes of G5P during bio-SAXS. The resulting microscopic energy-damage relation was determined by particle scattering simu- lations with TOPAS/Geant4. The results are interpreted in terms of radical scavenging as well as post-irradiation effects, related to preferential-exclusion from the protein surface. Thus, Ectoine provides an non-disturbing way to improve structure-determination of proteins via bio-SAXS in future studies.…
Autor*innen: | Marc Benjamin HahnORCiD |
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Weitere Person: | Dorothea C. Hallier, Glen Jacob SmalesORCiD, H. Seitz |
Dokumenttyp: | Posterpräsentation |
Veröffentlichungsform: | Präsentation |
Sprache: | Englisch |
Jahr der Erstveröffentlichung: | 2023 |
Organisationseinheit der BAM: | 6 Materialchemie |
6 Materialchemie / 6.6 Physik und chemische Analytik der Polymere | |
DDC-Klassifikation: | Naturwissenschaften und Mathematik / Chemie / Analytische Chemie |
Technik, Medizin, angewandte Wissenschaften / Ingenieurwissenschaften / Ingenieurwissenschaften und zugeordnete Tätigkeiten | |
Freie Schlagwörter: | Ectoin Bio-SAXS; BioSAXS; Compatible solute; Cosolute; DNA; Dosimetry; Ectoin; Ectoine; G5P; GVP; Geant4; Geant4-DNA; Gene five protein; Hydroxyectoine; Ionizing radiation damage; LEE; McSAS3; Median lethal energy deposit; Microdosimetry; Monte-Carlo simulations; OH Radical; OH radical scavenger; Osmolyte; Particle scattering simulations; Protein; Protein unfolding; Proteins; ROS; Radiation damage; Radical Scavenger; Radical scavenger; SAXS; Single-stranded DNA-binding proteins; Small-angle xray scattering; Topas; Topas-MC; Topas-nBio; X-ray scattering; ssDNA |
Themenfelder/Aktivitätsfelder der BAM: | Chemie und Prozesstechnik |
Material | |
Material / Nano | |
Veranstaltung: | MultiChem Conference 2023 |
Veranstaltungsort: | Prague, Czech Republic |
Beginndatum der Veranstaltung: | 26.04.2023 |
Enddatum der Veranstaltung: | 28.04.2023 |
Zugehöriger Identifikator: | https://doi.org/10.1039/D2CP05053F |
Verfügbarkeit des Dokuments: | Datei im Netzwerk der BAM verfügbar ("Closed Access") |
Datum der Freischaltung: | 04.05.2023 |
Referierte Publikation: | Nein |