F. Bertorelle, Karl David Wegner, M. P. Berkulic, H. Fakhouri, C. Comby-Zerbino, A. Sagar, P. Bernadó, Ute Resch-Genger, V. Bonacic-Koutecký, X. Le Guével, R. Antoine
- Atomically precise gold nanoclusters are a fascinating class of nanomaterials that exhibit molecule-like properties and have outstanding photoluminescence (PL). Their ultrasmall size, molecular chemistry, and biocompatibility make them extremely appealing for selective biomolecule labeling in investigations of biological mechanisms at the cellular and anatomical levels. In this work, we report a simple route to incorporate a preformed Au25 nanocluster into a model bovine serum albumin (BSA) protein. A new approach combining small-angle X-ray scattering and molecular modeling provides a clear localization of a single Au25 within the protein to a cysteine residue on the gold nanocluster surface.
Attaching Au25 to BSA strikingly modifies the PL properties with enhancement and a redshift in the second near-infrared (NIR-II) window. This study paves the way to conrol the design of selective sensitive probes in biomolecules through a ligand-based strategy to enable the optical detection ofAtomically precise gold nanoclusters are a fascinating class of nanomaterials that exhibit molecule-like properties and have outstanding photoluminescence (PL). Their ultrasmall size, molecular chemistry, and biocompatibility make them extremely appealing for selective biomolecule labeling in investigations of biological mechanisms at the cellular and anatomical levels. In this work, we report a simple route to incorporate a preformed Au25 nanocluster into a model bovine serum albumin (BSA) protein. A new approach combining small-angle X-ray scattering and molecular modeling provides a clear localization of a single Au25 within the protein to a cysteine residue on the gold nanocluster surface.
Attaching Au25 to BSA strikingly modifies the PL properties with enhancement and a redshift in the second near-infrared (NIR-II) window. This study paves the way to conrol the design of selective sensitive probes in biomolecules through a ligand-based strategy to enable the optical detection of biomolecules in a cellular environment by live imaging.…
MetadatenAutor*innen: | F. Bertorelle, Karl David WegnerORCiD, M. P. Berkulic, H. Fakhouri, C. Comby-Zerbino, A. Sagar, P. Bernadó, Ute Resch-GengerORCiD, V. Bonacic-Koutecký, X. Le Guével, R. Antoine |
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Dokumenttyp: | Zeitschriftenartikel |
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Veröffentlichungsform: | Verlagsliteratur |
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Sprache: | Englisch |
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Titel des übergeordneten Werkes (Englisch): | Chemistry - A European Journal |
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Jahr der Erstveröffentlichung: | 2022 |
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Organisationseinheit der BAM: | 1 Analytische Chemie; Referenzmaterialien |
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| 1 Analytische Chemie; Referenzmaterialien / 1.2 Biophotonik |
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Verlag: | Wiley-VCH |
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Verlagsort: | Weinheim |
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Jahrgang/Band: | 28 |
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Ausgabe/Heft: | 39 |
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Aufsatznummer: | e202200570 |
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Erste Seite: | 1 |
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Letzte Seite: | 8 |
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DDC-Klassifikation: | Naturwissenschaften und Mathematik / Chemie / Analytische Chemie |
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| Technik, Medizin, angewandte Wissenschaften / Ingenieurwissenschaften / Ingenieurwissenschaften und zugeordnete Tätigkeiten |
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Freie Schlagwörter: | Aggregation; Bioimaging; Cluster; Fluorescence; Gold; Imaging; Lifetime; Ligand; Metal; NIRII; Nano; Photophysics; Protein; Quantum yield; SWIR; Sensor; Signal enhancement; Synthesis |
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Themenfelder/Aktivitätsfelder der BAM: | Chemie und Prozesstechnik |
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| Material |
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| Material / Nano |
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| Umwelt |
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| Umwelt / Sensorik |
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DOI: | 10.1002/chem.202200570 |
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ISSN: | 1521-3765 |
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Verfügbarkeit des Dokuments: | Datei im Netzwerk der BAM verfügbar ("Closed Access") |
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Datum der Freischaltung: | 22.06.2022 |
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Referierte Publikation: | Ja |
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Datum der Eintragung als referierte Publikation: | 22.06.2022 |
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