- This paper discusses the feasibility of a novel strategy based on the combination of bioprinting nano-doping technology and laser ablation-inductively coupled plasma time-of-flight mass spectrometry analysis for the preparation and characterization of gelatin- based multi-element calibration standards suitable for quantitative imaging. To achieve this, lanthanide up-conversion nanoparticles were added to a gelatin matrix to produce the bioprinted calibration standards. The features of this bioprinting approach were com- pared with manual cryosectioning standard preparation, in terms of throughput, between batch repeatability and elemental signal homogeneity at 5 μm spatial resolution. By using bioprinting, the between batch variability for three independent standards of the same concentration of 89 Y (range 0–600 mg/kg) was reduced to 5% compared to up to 27% for cryosectioning. On this basis, the relative standard deviation ( RSD ) obtained between three independent calibration slopesThis paper discusses the feasibility of a novel strategy based on the combination of bioprinting nano-doping technology and laser ablation-inductively coupled plasma time-of-flight mass spectrometry analysis for the preparation and characterization of gelatin- based multi-element calibration standards suitable for quantitative imaging. To achieve this, lanthanide up-conversion nanoparticles were added to a gelatin matrix to produce the bioprinted calibration standards. The features of this bioprinting approach were com- pared with manual cryosectioning standard preparation, in terms of throughput, between batch repeatability and elemental signal homogeneity at 5 μm spatial resolution. By using bioprinting, the between batch variability for three independent standards of the same concentration of 89 Y (range 0–600 mg/kg) was reduced to 5% compared to up to 27% for cryosectioning. On this basis, the relative standard deviation ( RSD ) obtained between three independent calibration slopes measured within 1 day also reduced from 16% (using cryosectioning ) to 5% (using bioprinting), supporting the use of a single standard preparation replicate for each of the concentrations to achieve good calibration performance using bioprinting. This helped reduce the analysis time by approximately 3-fold. With cryosectioning each standard was prepared and sectioned individually, whereas using bio-printing it was possible to have up to six different standards printed simultaneously, reducing the preparation time from approximately 2 h to under 20 min (by approxi- mately 6-fold). The bio-printed calibration standards were found stable for a period of 2 months when stored at ambient temperature and in the dark.…
MetadatenAutor*innen: | K. Billimoria, Y. A. Diaz Fernandez, Elina AndresenORCiD, I. Sorzabal-Bellido, G. Huelga-Suarez, D. Bartczak, C. Ortiz de Solórzano, Ute Resch-GengerORCiD, H. Goenaga Infante |
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Dokumenttyp: | Zeitschriftenartikel |
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Veröffentlichungsform: | Verlagsliteratur |
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Sprache: | Englisch |
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Titel des übergeordneten Werkes (Englisch): | Metallomics |
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Jahr der Erstveröffentlichung: | 2022 |
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Organisationseinheit der BAM: | 1 Analytische Chemie; Referenzmaterialien |
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| 1 Analytische Chemie; Referenzmaterialien / 1.2 Biophotonik |
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Verlag: | Oxford University Press |
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Verlagsort: | Oxford |
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Jahrgang/Band: | 14 |
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Ausgabe/Heft: | 12 |
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Aufsatznummer: | mfac088 |
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Erste Seite: | 1 |
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Letzte Seite: | 9 |
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DDC-Klassifikation: | Naturwissenschaften und Mathematik / Chemie / Analytische Chemie |
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| Technik, Medizin, angewandte Wissenschaften / Ingenieurwissenschaften / Sanitär- und Kommunaltechnik; Umwelttechnik |
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Freie Schlagwörter: | 3D-printing; Environmental analysis; Fluorescence; LA-ICP-MS; Lanthanide; Multimodal; Nanoparticles; Production; Quality assurance; Reference material; Synthesis; Tag |
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Themenfelder/Aktivitätsfelder der BAM: | Chemie und Prozesstechnik |
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| Chemie und Prozesstechnik / Chemische Charakterisierung und Spurenanalytik |
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| Umwelt |
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| Umwelt / Umwelt-Material-Interaktionen |
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DOI: | 10.1093/mtomcs/mfac088 |
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ISSN: | 1756-591X |
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Verfügbarkeit des Dokuments: | Datei im Netzwerk der BAM verfügbar ("Closed Access") |
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Datum der Freischaltung: | 20.02.2023 |
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Referierte Publikation: | Ja |
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Datum der Eintragung als referierte Publikation: | 20.02.2023 |
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