Cerebral bio-imaging of Cu, Fe, Zn and Mn in the MPTP mouse model of Parkinsons disease using laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS)

  • Laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) has been established as a powerful technique for the determination of metal and nonmetal distributions within biological systems with high sensitivity. An imaging LA-ICP-MS technique for Fe, Cu, Zn, and Mn was developed to produce large series of quantitative element maps in native brain sections of mice subchronicallyLaser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) has been established as a powerful technique for the determination of metal and nonmetal distributions within biological systems with high sensitivity. An imaging LA-ICP-MS technique for Fe, Cu, Zn, and Mn was developed to produce large series of quantitative element maps in native brain sections of mice subchronically intoxicated with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridin (MPTP) as a model of Parkinson’s disease. Images were calibrated using matrix-matched laboratory standards. A software solution allowing a precise delineation of anatomical structures was implemented. Coronal brain sections were analyzed crossing the striatum and the substantia nigra, respectively. Animals sacrificed 2 h, 7 d, or 28 d after the last MPTP injection and controls were investigated. We observed significant decreases of Cu concentrations in the periventricular zone and the fascia dentata at 2 h and 7d and a recovery or overcompensation at 28 d, most pronounced in the rostral periventricular zone (+40%). In the cortex Cu decreased slightly to −10%. Fe increased in the interpeduncular nucleus (+40%) but not in the substantia nigra. This pattern is in line with a differential regulation of periventricular and parenchymal Cu, and with the histochemical localization of Fe, and congruent to regions of preferential MPTP binding described in the rodent brain. The LA-ICP-MS technique yielded valid and statistically robust results in the present study on 39 slices from 19 animals. Our findings underline the value of routine micro-local analytical techniques in the life sciences and affirm a role of Cu availability in Parkinson’s disease.show moreshow less

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Metadaten
Author:Andreas Matusch, Candan Depboylu, Christoph PalmORCiDGND, Bei Wu, Günter U. Höglinger, Martin K.-H. Schäfer, Johanna Sabine Becker
DOI:https://doi.org/10.1016/j.jasms.2009.09.022
Parent Title (English):Journal of the American Society for Mass Spectrometry
Document Type:Article
Language:English
Year of first Publication:2010
Release Date:2020/01/08
Tag:Inductively Couple Plasma Mass Spectrometry; Laser Ablation Inductively Couple Plasma Mass Spectrometry; MPTP; MPTP Treatment; Substantia Nigra
GND Keyword:ICP-Massenspektrometrie; Metalle; Gehirnkarte; MPTP
Volume:21
Issue:1
First Page:161
Last Page:171
Institutes:Fakultät Informatik und Mathematik
Fakultät Informatik und Mathematik / Labor Regensburg Medical Image Computing (ReMIC)
Publication:Externe Publikationen
research focus:Lebenswissenschaften und Ethik
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