New Approaches for the Quantification of Functional Groups on Micro- and Nanoparticle Surfaces

  • Nanometer- and micrometer-sized particles are increasingly used as tolls in (bio)analytics with typical applications being carriers for e.g., drugs or dye molecules for use as multichromophoric reporters for signal amplification in optical assays, platforms for DNA sequencing as well as nanosensors and targeted probes for bioimaging studies. The application of such particles in the material and life sciences is closely linked to their size (and size distribution), shape, colloidal stability, biocompatibility, and ease of subsequent functionalization, e.g., with linkers, targeting ligands, and sensor molecules. The latter requires knowledge of the number of groups effectively accessible for subsequent coupling reactions and hence, selective and sensitive methods of analysis. Ideally, these methods are be robust, reliable, fast, performable with inexpensive equipment, and can be employed for the characterization of a broad variety of particle systems independent of their opticalNanometer- and micrometer-sized particles are increasingly used as tolls in (bio)analytics with typical applications being carriers for e.g., drugs or dye molecules for use as multichromophoric reporters for signal amplification in optical assays, platforms for DNA sequencing as well as nanosensors and targeted probes for bioimaging studies. The application of such particles in the material and life sciences is closely linked to their size (and size distribution), shape, colloidal stability, biocompatibility, and ease of subsequent functionalization, e.g., with linkers, targeting ligands, and sensor molecules. The latter requires knowledge of the number of groups effectively accessible for subsequent coupling reactions and hence, selective and sensitive methods of analysis. Ideally, these methods are be robust, reliable, fast, performable with inexpensive equipment, and can be employed for the characterization of a broad variety of particle systems independent of their optical properties, i.e., scattering or the presence of encoding dyes. In this respect, we studied a variety of conventional and newly developed labels for optical readout on self-made particles with varying surface group density, utilizing e.g., changes in intensity and/or color of absorption and/or emission. We focus here on the development of a platform of cleavable and multimodal labels for optical assays which consist of a cleavable linker and an optically active reporter moiety. In contrast to conventional reporters measured directly at the particle surface, which are prone to signal distortions by scattering and encoding dyes, these cleavable labels can be detected colorimetrically or fluorometrically both bound at the particle surface and after quantitative cleavage of the linker in the transparent supernatant. Moreover, for heteroatom-containing reporters, they enable straightforward validation by method comparison with elemental analysis, ICP-OES or ICP-MS. Here, we present representative examples of our newly synthesized cleavable and multimodal labels and their application as reporters for the quantification of amino, thiol and carboxy surface groups on different nanomaterials and compare these results with measurements using conventional optical labels and results from measurements providing the total number of surface groups.zeige mehrzeige weniger

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Metadaten
Autor*innen:Nithiya Nirmalananthan-Budau
Koautor*innen:Marko Moser, Thomas Behnke, Ute Resch-Genger
Dokumenttyp:Vortrag
Veröffentlichungsform:Präsentation
Sprache:Englisch
Jahr der Erstveröffentlichung:2017
DDC-Klassifikation:Naturwissenschaften und Mathematik / Chemie / Analytische Chemie
Freie Schlagwörter:Nanoparticle; Optical quantification; Surface functional groups
Veranstaltung:ANAKON 2017
Veranstaltungsort:Tübingen, Germany
Beginndatum der Veranstaltung:03.04.2017
Enddatum der Veranstaltung:06.04.2017
Bemerkung:
Geburtsname von Nirmalananthan-Budau, Nithiya: Nirmalananthan, N. -  Birth name of Nirmalananthan-Budau, Nithiya: Nirmalananthan, N.
Verfügbarkeit des Dokuments:Datei im Netzwerk der BAM verfügbar ("Closed Access")
Datum der Freischaltung:12.04.2017
Referierte Publikation:Nein
Eingeladener Vortrag:Nein
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