Advances in high-resolution imaging of cell cultures and tissues exposed to airborne particles
- High-resolution microscopy techniques are fundamental to our ability to investigate and quantify the toxicological effects of airborne particulate pollutants via imaging of in vitro respiratory models exposed to well-characterised particle suspensions and aerosols. However, all imaging methods are limited in their spatio-temporal resolution, information capacity and suitability for minimally invasive time lapse imaging. Many traditional methods are incompatible with the more complex three-dimensional model systems which have potential for greater correlation between in vitro studies and real-world health effects. New techniques can overcome these limits, increasing our ability to understand and measure biological responses to particulate exposure at subcellular, cellular and multicellular scales. We have investigated a variety of microscopy methods for imaging different respiratory models.
Autor*innen: | C. Dondi |
---|---|
Koautor*innen: | S. Rey, Lena Scholtz, Isabella Tavernaro, Ute Resch-Genger, A-K. Larsson-Callerfelt, M. Shaw |
Dokumenttyp: | Posterpräsentation |
Veröffentlichungsform: | Präsentation |
Sprache: | Englisch |
Jahr der Erstveröffentlichung: | 2022 |
Organisationseinheit der BAM: | 1 Analytische Chemie; Referenzmaterialien |
1 Analytische Chemie; Referenzmaterialien / 1.2 Biophotonik | |
DDC-Klassifikation: | Technik, Medizin, angewandte Wissenschaften / Ingenieurwissenschaften / Sanitär- und Kommunaltechnik; Umwelttechnik |
Freie Schlagwörter: | Aerosol particles; Fluorescence microscopy; High-resolution imaging; In vitro imaging |
Themenfelder/Aktivitätsfelder der BAM: | Umwelt |
Umwelt / Sensorik | |
Umwelt / Umwelt-Material-Interaktionen | |
Veranstaltung: | International Aerosol Conference (IAC 2022) |
Veranstaltungsort: | Athens, Greece |
Beginndatum der Veranstaltung: | 04.09.2022 |
Enddatum der Veranstaltung: | 09.09.2022 |
Verfügbarkeit des Dokuments: | Datei im Netzwerk der BAM verfügbar ("Closed Access") |
Datum der Freischaltung: | 14.09.2022 |
Referierte Publikation: | Nein |