Characterization of biofilms with near ambient pressure XPS
- Near Ambient Pressure XPS opens up a new world of possibilities for measurements with XPS. While there are examples where NAP-XPS has been used to study electrochemical processes and heterogeneous catalysis, little attention has been paid to its potential use in biological materials. Until now, bacteria have only been characterised with conventional XPS, which requires tedious sample preparation usually involving freeze drying, a treatment that may degrade biological sample constituents. By studying biological samples in their native wet states, new insight about composition, absorption and transport of drugs through cell membranes and extracellular polymeric substance (EPS) layers can be obtained. Both artificial model-films of exopolysaccharides and biofilms of Escherichia Coli have been characterised at pressures ranging from ultra-high vacuum to 15 mbar by using SPECS’ EnviroESCA NAP-XPS instrument and conventional XPS. By applying antimicrobials to model biofilms, some of whichNear Ambient Pressure XPS opens up a new world of possibilities for measurements with XPS. While there are examples where NAP-XPS has been used to study electrochemical processes and heterogeneous catalysis, little attention has been paid to its potential use in biological materials. Until now, bacteria have only been characterised with conventional XPS, which requires tedious sample preparation usually involving freeze drying, a treatment that may degrade biological sample constituents. By studying biological samples in their native wet states, new insight about composition, absorption and transport of drugs through cell membranes and extracellular polymeric substance (EPS) layers can be obtained. Both artificial model-films of exopolysaccharides and biofilms of Escherichia Coli have been characterised at pressures ranging from ultra-high vacuum to 15 mbar by using SPECS’ EnviroESCA NAP-XPS instrument and conventional XPS. By applying antimicrobials to model biofilms, some of which are known to be resistant towards the antimicrobial in question, the distribution of antimicrobials in biofilms has been studied. Capabilities and limitations of the approach will be discussed.…
Autor*innen: | Marit Kjaervik |
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Koautor*innen: | Karin Schwibbert, Wolfgang UngerORCiD, S. Bahr, M. Meyer, P. Dietrich, A. Thissen |
Dokumenttyp: | Vortrag |
Veröffentlichungsform: | Präsentation |
Sprache: | Englisch |
Jahr der Erstveröffentlichung: | 2017 |
DDC-Klassifikation: | Naturwissenschaften und Mathematik / Chemie / Analytische Chemie |
Freie Schlagwörter: | Biofilms; E. coli; NAP-XPS |
Veranstaltung: | The 4th annual workshop on ambient presssure X-ray photoelectron spectroscopy |
Veranstaltungsort: | Shanghai, China |
Beginndatum der Veranstaltung: | 13.12.2017 |
Enddatum der Veranstaltung: | 15.12.2017 |
Verfügbarkeit des Dokuments: | Datei im Netzwerk der BAM verfügbar ("Closed Access") |
Datum der Freischaltung: | 02.01.2018 |
Referierte Publikation: | Nein |
Eingeladener Vortrag: | Nein |