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Standardization of fluorescence measurements: Design, development, validation, and fabrication of format-adaptable fluorescence standards for intensity, spectral, and temporal quantities

  • Photoluminescence techniques are amongst the most widely used tools in the material and life sciences, with new and exciting applications continuously emerging, due to their many advantages like comparative ease of use, unique sensitivity, non-invasive character, and potential for multiplexing, remote sensing, and miniaturization. Drawbacks are , however, signals, that contain unwanted wavelength- and polarization contributions from instrument-dependent effects, which are time-dependent due to the aging of instrument components, and difficulties to measure absolute fluorescence intensities. Thus, there is a considerable need for standards for intensity, spectral, and temporal fluorescence quantities to meet the increasing need for instrument performance validation and global trends to harmonize physicochemical measurements. In this respect, instrument calibration strategies together with different types of fluorescence standards are presented as well as design concepts for robust,Photoluminescence techniques are amongst the most widely used tools in the material and life sciences, with new and exciting applications continuously emerging, due to their many advantages like comparative ease of use, unique sensitivity, non-invasive character, and potential for multiplexing, remote sensing, and miniaturization. Drawbacks are , however, signals, that contain unwanted wavelength- and polarization contributions from instrument-dependent effects, which are time-dependent due to the aging of instrument components, and difficulties to measure absolute fluorescence intensities. Thus, there is a considerable need for standards for intensity, spectral, and temporal fluorescence quantities to meet the increasing need for instrument performance validation and global trends to harmonize physicochemical measurements. In this respect, instrument calibration strategies together with different types of fluorescence standards are presented as well as design concepts for robust, easy-to-use, and format-adaptable fluorescence standards useable for the determination of different fluorescence parameters and a broad variety of fluorescence techniques.zeige mehrzeige weniger

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  • UR_SALSA_Standardization of Fluorescence Measurements_0507_2016.pdf
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Metadaten
Autor*innen:Ute Resch-GengerORCiD
Dokumenttyp:Vortrag
Veröffentlichungsform:Präsentation
Sprache:Englisch
Jahr der Erstveröffentlichung:2016
DDC-Klassifikation:Naturwissenschaften und Mathematik / Chemie / Analytische Chemie
Technik, Medizin, angewandte Wissenschaften / Ingenieurwissenschaften / Ingenieurwissenschaften und zugeordnete Tätigkeiten
Freie Schlagwörter:Fluorescence; Quality assurance; Quantum yield; Spectral correction; Standard
Veranstaltung:SALSA-Kolloquien
Veranstaltungsort:Berlin, Germany
Beginndatum der Veranstaltung:07.06.2016
Verfügbarkeit des Dokuments:Datei im Netzwerk der BAM verfügbar ("Closed Access")
Datum der Freischaltung:04.08.2016
Referierte Publikation:Nein
Eingeladener Vortrag:Nein
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