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Quantum dots increasingly gain popularity for in vivo applications. However, their delivery and accumulation into cells can be challenging and there is still lack of detailed information. Thereby, the application of advanced fluorescence techniques can expand the portfolio of useful parameters for a more comprehensive evaluation. Here, we encapsulated hydrophilic quantum dots into liposomes for studying cellular uptake of these so-called lipodots into living cells. First, we investigated photophysical properties of free quantum dots and lipodots observing changes in the fluorescence decay time and translational diffusion behaviour. In comparison to empty liposomes, lipodots exhibited an altered zeta potential, whereas their hydrodynamic size did not change. Fluorescence lifetime imaging microscopy (FLIM) and fluorescence correlation spectroscopy (FCS), both combined with two-photon excitation (2P), were used to investigate the interaction behaviour of lipodots with an insect epithelial tissue. In contrast to the application of free quantum dots, their successful delivery into the cytosol of salivary gland duct cells could be observed when applying lipodots. Lipodots with different lipid compositions and surface charges did not result in considerable differences in the intracellular labelling pattern, luminescence decay time and diffusion behaviour. However, quantum dot degradation after intracellular accumulation could be assumed from reduced luminescence decay times and blue-shifted luminescence signals. In addition to single diffusing quantum dots, possible intracellular clustering of quantum dots could be assumed from increased diffusion times. Thus, by using a simple and manageable liposome carrier system, 2P-FLIM and 2P-FCS recording protocols could be tested, which are promising for investigating the fate of quantum dots during cellular interaction.
Forschungsbericht 2018
(2019)
Femtosecond-Pulsed Laser Written and Etched Fiber Bragg Gratings for Fiber-Optical Biosensing
(2018)
We present the development of a label-free, highly sensitive fiber-optical biosensor for online detection and quantification of biomolecules. Here, the advantages of etched fiber Bragg gratings (eFBG) were used, since they induce a narrowband Bragg wavelength peak in the reflection operation mode. The gratings were fabricated point-by-point via a nonlinear absorption process of a highly focused femtosecond-pulsed laser, without the need of prior coating removal or specific fiber doping. The sensitivity of the Bragg wavelength peak to the surrounding refractive index (SRI), as needed for biochemical sensing, was realized by fiber cladding removal using hydrofluoric acid etching. For evaluation of biosensing capabilities, eFBG fibers were biofunctionalized with a single-stranded DNA aptamer specific for binding the C-reactive protein (CRP). Thus, the CRP-sensitive eFBG fiber-optical biosensor showed a very low limit of detection of 0.82 pg/L, with a dynamic range of CRP detection from approximately 0.8 pg/L to 1.2 µg/L. The biosensor showed a high specificity to CRP even in the presence of interfering substances. These results suggest that the proposed biosensor is capable for quantification of CRP from trace amounts of clinical samples. In addition, the adaption of this eFBG fiber-optical biosensor for detection of other relevant analytes can be easily realized.
Forschungsbericht 2017
(2018)
Für beruflich Qualifizierte ist der Weg in die Hochschulen und Universitäten in den letzten Jahren einfacher geworden. Die heterogenen Wege der beruflich Qualifizierten ins Studium werden wir in diesem Artikel beleuchten und aufzeigen, wie wir in unseren Mathematikvorkursen an die Vorkenntnisse unserer Studieninteressierten anknüpfen. Eine unter beruflich qualifizierten Studieninteressierten durchgeführte Befragung soll mehr Aufschluss darüber geben, über welche unterschiedlichen Wege und aus welchen persönlichen Gründen beruflich Qualifizierte ein Studium aufnehmen. Da Mathematik im Studium gerade für beruflich Qualifizierte meist eine große Hürde darstellt, hat die Technische Hochschule Wildau ihr Vorkursangebot auf die heterogener werdende Studierendenschaft angepasst.
Forschungsbericht 2016
(2017)
Die vorliegende Festschrift ist eine farbenfrohe Sammlung von Beiträgen anlässlich des 10-jährigen Bestehens unserer Technischen Fachhochschule Wildau. Autoren sind Mitglieder und Angehörige, Freunde und Förderer der Technischen Fachhochschule Wildau, Vertreter von in- und ausländischen Partnerhochschulen, von Praxispartnern aus der Wirtschaft und der öffentlichen Verwaltung unserer Hochschule. Die Form der Beiträge weist auch eine breite Skala von Grußworten über persönliche Erinnerungen bis hin zu berichtsähnlichen Aufsätzen auf.