Characterization of dye-protein conjugates with optical methods
- Fluorophore labeled proteins and antibodies, referred to also as targeted optical probes, present a promising strategy for a variety of applications from fundamental cell-based biological studies to in vivo diagnostics and image guided surgeries in humans. In this respect, design strategies for the preparation of such conjugates from different dyes including analyte-responsive fluorophores are presented as well as their analytical and spectroscopic characterization employing Absorption spectroscopy and steady state and time-resolved fluorometry. Special emphasis is dedicated to the influence of dye hydrophilicity and labeling density on the optical properties and binding behavior of these dye-bioconjugates including their performance in in vitro and in vivo bioimaging studies.
Autor*innen: | Ute Resch-GengerORCiD |
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Koautor*innen: | Jutta Pauli |
Dokumenttyp: | Vortrag |
Veröffentlichungsform: | Präsentation |
Sprache: | Englisch |
Jahr der Erstveröffentlichung: | 2016 |
DDC-Klassifikation: | Naturwissenschaften und Mathematik / Chemie / Analytische Chemie |
Technik, Medizin, angewandte Wissenschaften / Ingenieurwissenschaften / Sanitär- und Kommunaltechnik; Umwelttechnik | |
Freie Schlagwörter: | Dye; Dye bioconjugates; Fluorescence; Imaging; Immunoassay; NIR; Optical characterization; Protein; Quality assurance |
Veranstaltung: | International Workshop “Affinity - Mass spectrometry – New Methods and Application to Protein Therapeutics Development” |
Veranstaltungsort: | Rüsselsheim am Main, Germany |
Beginndatum der Veranstaltung: | 14.11.2016 |
Enddatum der Veranstaltung: | 15.11.2016 |
Verfügbarkeit des Dokuments: | Datei im Netzwerk der BAM verfügbar ("Closed Access") |
Datum der Freischaltung: | 30.11.2016 |
Referierte Publikation: | Nein |
Eingeladener Vortrag: | Nein |