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Zinc distribution in human bone: Sr-micro X-ray fluorescence imaging of osteoporotic samples

  • Zn is known to be located in the reactive centers of various enzymes, which play a major role in the mineralization process at sites where new bone formation occurs. In addition, elevated Zn levels are supposed to increase the proliferation rate of osteoblasts [1] and may lead to a stimulation of bone formation in vitro and in vivo [2]. Consequently, Zn seems to play an essential role in bone formation and mineralization through various pathways. We thus expected Zn levels to be altered at sites of extensive bone formation like in the case of fracture healing. We measured the same areas on human bone samples with both a scanning confocal synchrotron radiation induced micro X-ray fluorescence (SR-μXRF) at the FLUO beamline (ANKA) and a full-field Color X-ray Camera at the BAMline (Bessy II) setup in order to find the ideal SR-μXRF imaging method to investigate trace element distributions in bone samples. As zinc is a trace element of special interest in bone, the setups were optimizedZn is known to be located in the reactive centers of various enzymes, which play a major role in the mineralization process at sites where new bone formation occurs. In addition, elevated Zn levels are supposed to increase the proliferation rate of osteoblasts [1] and may lead to a stimulation of bone formation in vitro and in vivo [2]. Consequently, Zn seems to play an essential role in bone formation and mineralization through various pathways. We thus expected Zn levels to be altered at sites of extensive bone formation like in the case of fracture healing. We measured the same areas on human bone samples with both a scanning confocal synchrotron radiation induced micro X-ray fluorescence (SR-μXRF) at the FLUO beamline (ANKA) and a full-field Color X-ray Camera at the BAMline (Bessy II) setup in order to find the ideal SR-μXRF imaging method to investigate trace element distributions in bone samples. As zinc is a trace element of special interest in bone, the setups were optimized for Zn detection. The setups were compared concerning count rate, required measurement time and resolution. We could show that the ideal method is depending on the element of interest. While for Ca (a major constituent of the bone with a low energy of 3.69keV for K) the Color X-ray Camera provided us with a higher resolution in the plane, for Zn (a trace element in bone) only the confocal SR-μXRF was able to sufficiently image the distribution. Biopsies of healing osteoporotic fractures (Vertebral compression fractures (VCFs)) were investigated in regard to their Zn distribution. The samples were measured with a confocal SR-μXRF setup with a 10 μm x 15 μm resolution at the FLUO beamline at ANKA. As we found increased Zn levels, which seemed to be accumulated in narrow structures between bone packages we also investigated thin cuts (4 μm thick) of two sample areas with a higher resolution of 1 μm x 1 μm (monochromatic beam with E= 17 keV) at B16 at Diamond SR facility. We will present the advantages and disadvantages of all three SR-μXRF setups (ANKA FLUO beamline, Bessy II BAMline, and Diamond B16) for imaging elemental distributions in bone with a focus on Zn. We will also show the distribution of Zn in healing VCFs.zeige mehrzeige weniger

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Metadaten
Autoren/innen:M. Rauwolf
Koautoren/innen:A. Turyanskaya, A. Roschger, J. Prost, R. Simon, I. Pape, Martin Radtke, O. Scharf, Tom Schoonjans, Ana de Oliveira Guilherme Buzanich, K. Sawhney, P. Wobrauschek, P. Rocshger, J.G. Hofstaetter, C. Streli
Dokumenttyp:Posterpräsentation
Veröffentlichungsform:Präsentation
Sprache:Englisch
Jahr der Erstveröffentlichung:2016
Organisationseinheit der BAM:1 Analytische Chemie; Referenzmaterialien
1 Analytische Chemie; Referenzmaterialien / 1.3 Strukturanalytik
DDC-Klassifikation:Naturwissenschaften und Mathematik / Chemie / Analytische Chemie
Freie Schlagwörter:BAMline; Synchrotron; XRF
Themenfelder/Aktivitätsfelder der BAM:Analytical Sciences
Analytical Sciences / Spurenanalytik und chemische Zusammensetzung
Veranstaltung:XRM2016: 13th International Conference on X-Ray Microscopy
Veranstaltungsort:Oxford, UK
Beginndatum der Veranstaltung:15.08.2016
Enddatum der Veranstaltung:19.08.2016
Verfügbarkeit des Volltexts:Volltext-PDF im Netzwerk der BAM verfügbar ("Closed Access")
Datum der Freischaltung:21.12.2016
Referierte Publikation:Nein