Lifetime-encoded microparticles for application in flow cytometry
- The steadily increasing number of analytes, especially in bioanalytics and environmental contexts, requires the development of efficient and versatile methods for the simultaneous determination of different parameters within a single run. Additionally, these methods should be applicable in conjunction with established platform technologies like flow cytometry. Fluorescence techniques have proven to fulfill these requirements. Commonly performed spectral multiplexing utilizing a color code suffers from several problems, such as the sensitivity of fluorescence intensity measurements to fluctuations in excitation light intensity and dye concentration and hence, photobleaching and spectral crosstalk limiting the achievable number of detection channels. Moreover, it typically requires different costly excitation light sources.
Autor*innen: | Daniel Kage |
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Koautor*innen: | Katrin Hoffmann, M. Wittkamp, J. Ameskamp, W. Göhde, T. Thiele, U. Schedler, Ute Resch-Genger |
Dokumenttyp: | Posterpräsentation |
Veröffentlichungsform: | Präsentation |
Sprache: | Englisch |
Jahr der Erstveröffentlichung: | 2016 |
DDC-Klassifikation: | Naturwissenschaften und Mathematik / Chemie / Analytische Chemie |
Freie Schlagwörter: | flow cytometry; fluorescence; life sciences; lifetime encoding; polymer particles |
Veranstaltung: | Innovation Forum Senftenberg 2016 |
Veranstaltungsort: | Senftenberg, Germany |
Beginndatum der Veranstaltung: | 01.06.2016 |
Enddatum der Veranstaltung: | 02.06.2016 |
Verfügbarkeit des Dokuments: | Datei im Netzwerk der BAM verfügbar ("Closed Access") |
Datum der Freischaltung: | 23.08.2016 |
Referierte Publikation: | Nein |