Detection of aluminum nanoparticles in biological media and in vitro
- Aluminum is the third most abundant element in the earth crust and therefore ubiquitously detectable in the environment. Mostly found in the form of derivatives such as silicates or oxides, it also occurs as metallic aluminum for example as colorant in sweets or in aluminum foil. With regard to potential toxicological effects, the different solubility of metallic aluminum nanoparticles compared to Al2O3 is of high relevance. Formation of ions may facilitate the crossing of blood-tissue barriers. Distribution towards other organs and subsequent re-formation of particulate aluminum due to milieu changes might occur. Therefore, the determination of solubility is required for proper risk assessment. Inductively coupled plasma mass spectrometry (ICP-MS) allows determination of aluminum with a detection limit of about 6 ppb. It could be proven that dissolution and solubility of metallic aluminum is significantly different when compared to Al2O3. Using ICP-MS in the single particle mode,Aluminum is the third most abundant element in the earth crust and therefore ubiquitously detectable in the environment. Mostly found in the form of derivatives such as silicates or oxides, it also occurs as metallic aluminum for example as colorant in sweets or in aluminum foil. With regard to potential toxicological effects, the different solubility of metallic aluminum nanoparticles compared to Al2O3 is of high relevance. Formation of ions may facilitate the crossing of blood-tissue barriers. Distribution towards other organs and subsequent re-formation of particulate aluminum due to milieu changes might occur. Therefore, the determination of solubility is required for proper risk assessment. Inductively coupled plasma mass spectrometry (ICP-MS) allows determination of aluminum with a detection limit of about 6 ppb. It could be proven that dissolution and solubility of metallic aluminum is significantly different when compared to Al2O3. Using ICP-MS in the single particle mode, a significant change in the behavior of both aluminum species was detected after undergoing the artificial digestion. Nearly unchanged in the saliva, particles show dissolution and high agglomeration during the gastric state before deagglomerating again in the intestine. Further analysis by time-of-flight secondary ion mass spectrometry (ToF-SIMS) revealed the uptake of both aluminum forms by proliferating and differentiated Caco-2 cells. For both particle forms different ions could be detected. Several aluminum-amino acid complex-derived ions from serine and valine were identified. In the case of Al2O3, Al2O2+, AlOH+, AlH2O+ and Al[(H2O)6]3+ were the main ions found co-localizing within treated cells.…
Autor*innen: | B. Krause |
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Koautor*innen: | H. Sieg, Claudia Kästner, T. Meyer, P. Reichardt, D. Lichtenstein, A. Burel, L. Boehmert, H. Jungnickel, J. Tentschert, P. Laux, A. Braeuning, I. Estrela-Lopis, F. Gauffre, V. Fessard, Andreas Thuenemann, A. Lampen, A. Luch |
Dokumenttyp: | Posterpräsentation |
Veröffentlichungsform: | Präsentation |
Sprache: | Englisch |
Jahr der Erstveröffentlichung: | 2017 |
DDC-Klassifikation: | Naturwissenschaften und Mathematik / Chemie / Analytische Chemie |
Freie Schlagwörter: | Aluminum; Artificial digestion; Cellular uptake; SAXS; SP-ICP-MS |
Veranstaltung: | European Winter Conference for Plasma Spectrochemistry 2017 |
Veranstaltungsort: | Sankt Anton, Austria |
Beginndatum der Veranstaltung: | 19.02.2017 |
Enddatum der Veranstaltung: | 24.02.2017 |
Verfügbarkeit des Dokuments: | Datei im Netzwerk der BAM verfügbar ("Closed Access") |
Datum der Freischaltung: | 20.02.2017 |
Referierte Publikation: | Nein |