4 Material und Umwelt
Filtern
Dokumenttyp
- Vortrag (27)
- Posterpräsentation (12)
- Zeitschriftenartikel (4)
- Beitrag zu einem Tagungsband (2)
- Sonstiges (1)
Referierte Publikation
- nein (46) (entfernen)
Schlagworte
- Indoor air quality (5)
- Single cell analysis (5)
- Microbiologically influenced corrosion (4)
- Sc-ICP-ToF-MS (4)
- VOC (4)
- Archaea (3)
- Emission reference materials (3)
- Materials emissions test (3)
- SC-ICP-ToF-MS (3)
- Umweltsimulation (3)
- Affinity chromatography (2)
- Antibodies (2)
- Bundesoberbehörden (2)
- Carbon steel corrosion (2)
- Combustion Ion Chromatography (2)
- Electron microscopy (2)
- Environmental simulation (2)
- Fluorescence (2)
- Glutaraldehyde (2)
- Ir DNA staining approach (2)
- Iron oxide nanoparticles (2)
- LA-ICP-MS (2)
- Method development (2)
- Microbiological influenced corrosion MIC (2)
- Nano (2)
- Nanomaterial (2)
- Nanomaterialien (2)
- Particle size (2)
- Per- and Polyfluoroalkyl substances (PFAS) (2)
- Pollutants (2)
- Polyglycerol (2)
- Reductive amination (2)
- Reference material (2)
- Schadstoffaustrag (2)
- Sewage sludge (2)
- Single cell (2)
- Size (2)
- 3D printing (1)
- Additive Fertigung (1)
- Additive manufacturing (1)
- Adsorbable organically bound fluorine (AOF) (1)
- Advanced Materials (1)
- Affinity extraction (1)
- Affinity support (1)
- Aluminum oxide (1)
- Amino acid analysis (1)
- Ammoniak (1)
- Analytical standards (1)
- Antibody purification (1)
- Aromatic amino acid analysis AAAA (1)
- Aromatic amino acid analysis aaaa (1)
- BAM Data Store (1)
- BFR (1)
- BODIPY dye (1)
- BSA (1)
- Bewitterung (1)
- Bioconjugation (1)
- Biofilm (1)
- Biokorrosion (1)
- Bioseparation (1)
- Bovine serum albumin (1)
- Böden (1)
- Carrier (1)
- Chamber test (1)
- Chamber-test (1)
- Chemical composition (1)
- Combustion ion chromatography (CIC) (1)
- Core/shell nanoparticle (1)
- Cubical shape (1)
- Downstream processing (1)
- E. coli (1)
- ESEM (1)
- Electronic lab notebook (ELN) (1)
- Elektrolichtbogenofen (1)
- Elektronisches Laborbuch (1)
- Emission reference material (1)
- Emission testing (1)
- Extractable organically bound fluorine (EOF) (1)
- Fertilizer (1)
- Flammschutzmittel (1)
- Fluorometric sensing (1)
- Food safety (1)
- Forschungsdatenmanagement (1)
- Forschungsstrategie (1)
- Gas sensing (1)
- HR-CS-GFMAS (1)
- High resolution-continuum source-graphite furnace molecular absorption spectrometry (HR-CS-GFMAS) (1)
- Human plasma (1)
- Hydrogenasen (1)
- Hyperbranched polymer (1)
- ISO/TC 229 Nanotechnologies (1)
- Igg (1)
- Immunoglobulins (1)
- Immunoprecipitation (1)
- Iron oxide (1)
- Leaching (1)
- Lightning talk (1)
- Linker (1)
- MDG ICP-ToF-MS (1)
- Materials emissions testing (1)
- Microbial (1)
- Microbially Induced Corrosion (1)
- Microdroplet generator (1)
- Microfluidics (1)
- Migration (1)
- Mikrobiell (1)
- Multigassensorik (1)
- Mycotoxins (1)
- NDT (1)
- Nano CRM (1)
- Nano@BAM (1)
- NanoPlattform (1)
- Nanocarrier (1)
- Nanoparticles (1)
- Nanopartikel (1)
- Nanoplattform (1)
- Nanotechnologie (1)
- Neuartige Materialien (1)
- Odour (1)
- OpenBIS (1)
- PBFSM (1)
- PFAS (1)
- PFC (1)
- PP (1)
- PS (1)
- Particles (1)
- Partikeln (1)
- Per- and polyfluorinated alkyl substances (PFASs) (1)
- Perceived intensity (1)
- Periodate oxidation (1)
- Perowskit (1)
- Phosphonic acids (1)
- Poficiency test (1)
- Polypropylen (1)
- Polystyrol (1)
- Poster presentation (1)
- ProMoAM (1)
- Process monitoring (1)
- Proficiency Test (1)
- Protein (1)
- Protein a (1)
- Protein hydrolysis (1)
- Protein immobilization (1)
- Protein quantification (1)
- Prozessmonitoring (1)
- Purification (1)
- Quality assurance/quality control (1)
- Quantum dot (1)
- RFA (1)
- Recycling (1)
- Reduktionsprozess (1)
- Reference data (1)
- Reference materials (1)
- Reference nanoparticles (1)
- Reference procedures (1)
- Release kinetics (1)
- Research data management (1)
- SAXS (1)
- SDS-PAGE (1)
- Sapphire (1)
- Sediment (1)
- Self-assembled monolayer (SAM) (1)
- Sensor (1)
- Small angle x-ray scattering (1)
- Smartphone (1)
- Soil (1)
- Solid phase (1)
- Spectroscopy (1)
- Surface chemistry (1)
- Surfacewaters (1)
- TEM (1)
- Tantal (1)
- Test strip (1)
- Teststreifen (1)
- Thermal imaging (1)
- Transmission electron microscopy (1)
- Tyrosine (1)
- Ultrafine particles (1)
- VOC-Emission (1)
- VOC-emission (1)
- Velocimetry (1)
- Weathering (1)
- XRF (1)
Organisationseinheit der BAM
- 1 Analytische Chemie; Referenzmaterialien (46) (entfernen)
Eingeladener Vortrag
- nein (27)
Monodisperse iron oxide nanoparticles as reference material candidate for particle size measurements
(2020)
In order to utilize and rationally design materials at the nanoscale the reliable characterization of their physico-chemical properties is highly important, especially with respect to the assessment of their environmental or biological impact. Furthermore, the European Commission’s REACH Regulations require the registration of nanomaterials traded in quantities of at least 1 ton. Powders or dispersions where 50% (number distribution) of the constituent particles have sizes ≤ 100 nm in at least one dimension are defined as nanomaterials. This creates a need for industrial manufacturers and research or analytical service facilities to reliably characterize potential nanomaterials. Currently, BAM is developing reference nanoparticles, which shall expand the scarce list of worldwide available nano reference materials certified for particle size distribution and will also target other key parameters like shape, structure, porosity or functional properties. In this respect, materials like iron oxide or titanium dioxide are considered as candidates to complement the already available silica, Au, Ag, and polystyrene reference nanoparticles.
The thermal decomposition of iron oleate precursors in high boiling organic solvents can provide large quantities of iron oxide nanoparticles that can be varied in size and shape.[1, 2] The presence of oleic acid or other hydrophobic ligands as capping agents ensures stable dispersion in nonpolar solvents. Such monodisperse, spherical particles were synthesized at BAM and pre-characterized by electron microscopy (TEM, SEM including the transmission mode STEM-in-SEM) and dynamic light scattering comparing cumulants analysis and frequency power spectrum.
1. REACH regulations and nanosafety concerns create a strong need for nano reference materials with diverse properties.
2. Iron oxide nanoparticles are under development as new candidate reference material at BAM.
3. Narrow particle size distribution confirmed by light scattering and electron microscopy.
The project ProMoAM is presented. The goal of the project is to evaluate which NDT techniques or combination of techniques is suited for in-situ quality assurance in additive manufacturing of metals. To this end, also 3d-data fusion and visualization techniques are applied. Additional ex-situ NDT-techniques are used as references for defect detection and quantification. Feasability studies for NDT-techniques that are presently not applicable for in-situ use are performed as well.
The presentation gives a brief overview of the whole project and the different involved NDT-techniques.
Fluorometric sensing is a versatile approach for trace analysis outside of the laboratory, requiring suitable sensor materials and their integration into sensing devices. The versatility of fluorophores as probes, especially in terms of the possibility to tailor their optical as well as their recognition properties by synthetic modifications in a wide range, renders them as superior active component for the preparation of optical sensing devices. Recent works at BAM in this field include, for example, the detection of nerve gas agents, illustrating impressively the aforementioned benefits of fluorophores in optical sensing applications.
In the interdisciplinary project presented here, we target hazardous gases such as ammonia, benzene, and hydrogen sulfide, next to others, which pose a major threat to human health and environmental safety and for which the availability of a sensitive and reliable detection method is highly desirable. The dyes presented follow a “turn-on” fluorescence schematic, which allows for the selective and sensitive detection of the respective gaseous analyte. The immobilization of the probe in polymeric matrices is then the next step toward the fabrication of a prototype device for molecular sensing. Further steps in the project include the assembly of instruments for test-atmosphere generation, the referencing of the sensor system, development and implementation of an optical setup, and the testing of the prototype device under laboratory conditions and in the field.
In this presentation, we give an overview over the recent developments on this topic in our groups. Highlights are hydrogen sulfide sensitive, BODIPY based transition metal complexes, which allow for a sensitive as well as selective detection of the toxic gas. In addition, we present a novel class of highly substituted BODIPY derivatives – pocket-BODIPYs – which are of a synthetically high versatility and can readily be modified to create pockets in the periphery of the molecule of defined geometries. This is illustrated on the successful encapsulation of benzene by a pocket-BODIPY derivative, confirmed by X-ray crystallographic analysis as well as by further spectroscopic and analytical methods.
Microbial contamination of fuels by fungi or bacteria poses risks such as corrosion and fuel system fouling, which can lead to critical problems in refineries and distribution systems and has a significant economic impact at every stage of the process. Many factors have been cited as being responsible for microbial growth, like the presence of water in the storage tanks. In fact, only 1 % water in a storage system is sufficient for the growth of microorganisms like bacteria or yeasts, as well as for the development of fungal biomass at the oil/water interface.
This work presents a rapid test for the accurate determination of genomic DNA from aqueous fuel extracts. The detection is based on the use of polystyrene-mesoporous silica core-shell particles onto which modified fluorescent molecular beacons are covalently grafted. These beacons contain in the hairpin loop a target sequence highly conserved in all bacteria, corresponding to a fragment of the 16S ribosomal RNA subunit. The designed single-stranded molecular beacon contained fluorescein as an internal indicator and a quencher in its proximity when not hybridized. Upon hybridization in presence of the target sequence, the indicator and the quencher are spatially separated, resulting in fluorescence enhancement. To perform the assay the developed particles were deposited on different glass fibre strips to obtain a portable and sensitive rapid test. The assays showed that the presence of genomic DNA extracts from bacteria down to 50–70 μg L–1 induced a fluorescence response. The optical read-out was adapted for on-site monitoring by fitting a 3D-printed case to a conventional smartphone, taking advantages of the sensitivity of the CMOS detector. Such embedded assembly enabled the detection of genomic DNA in aqueous extracts down to the mg L–1 range and represents an interesting step toward on-site monitoring of fuel contamination.
The biogenesis of iron–sulfur (Fe/S) proteins entails the synthesis and trafficking of Fe/S clusters, followed by their insertion into target apoproteins. In eukaryotes, the multiple steps of biogenesis are accomplished by complex protein machineries in both mitochondria and cytosol. The underlying biochemical pathways have been elucidated over the past decades, yet the mechanisms of cytosolic [2Fe-2S] protein assembly have remained ill-defined. Similarly, the precise site of glutathione (GSH) requirement in cytosolic and nuclear Fe/S protein biogenesis is unclear, as is the molecular role of the GSH-dependent cytosolic monothiol glutaredoxins (cGrxs). Here, we investigated these questions in human and yeast cells by various in vivo approaches. [2Fe-2S] cluster assembly of cytosolic target apoproteins required the mitochondrial ISC machinery, the mitochondrial transporter Atm1/ABCB7 and GSH, yet occurred independently of both the CIA system and cGrxs. This mechanism was strikingly different from the ISC-, Atm1/ABCB7-, GSH-, and CIA-dependent assembly of cytosolic–nuclear [4Fe-4S] proteins. One notable exception to this cytosolic [2Fe-2S] protein maturation pathway defined here was yeast Apd1 which used the CIA system via binding to the CIA targeting complex through its C-terminal tryptophan. cGrxs, although attributed as [2Fe-2S] cluster chaperones or trafficking proteins, were not essential in vivo for delivering [2Fe-2S] clusters to either CIA components or target apoproteins. Finally, the most critical GSH requirement was assigned to Atm1-dependent export, i.e. a step before GSH-dependent cGrxs function. Our findings extend the general model of eukaryotic Fe/S protein biogenesis by adding the molecular requirements for cytosolic [2Fe-2S] protein maturation.
Tour de table - BAM
(2021)
Die gemeinsame Forschungsstrategie der Bundesoberbehörden zur Nanotechnologie wurde 2016 veröffentlicht. Die darin enthaltenen Aufgaben wurden von den Bundesoberbehörden vielfältig bearbeitet. Diese Präsentation gibt einen Überblick über die Projekte, die von der BAM bis 2019 bearbeitet wurden/werden und sich in den Rahmen der Forschungsstrategie einordnen.
With the latest ICP-MS technology - ICP-ToF (time of flight)-MS - it is possible to analyze the multi-element fingerprint of individual cells. The interface between material and environmental analysis thus receives special attention, e.g. when considering corrosion processes. Microbiologically influenced corrosion (MIC) is highly unpredictable due to the diversity of microbial communities involved. The development of the MIC-specific ICP-ToF-MS analytical method presented here at the single cell level, in combination with the investigation of steel-MIC interactions, contributes significantly to progress in instrumental MIC analysis and will enable clarification of the processes taking place.
The supplied document shows the basis of a four minutes lightning talk.