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Quantification of ethyl glucuronide in hair: effect of milling on extraction efficiency

  • Aim: The objective of the study was to provide conclusive evidence for the effect of particle size reduction as by milling on the extractable content of ethyl glucuronide (EtG) of hair samples. Methods: A number of real case hair samples and two pooled hair materials with EtG contents in the range of 10–30 pg/mg were systematically compared with regard to the extraction yield of EtG after cutting to 2–3 mm length and pulverization with a ball mill. After the respective treatment the samples were submitted to aqueous extraction followed by quantification of EtG using HPLC-MS/MS. Results: It was unequivocally demonstrated that milling of hair samples prior to aqueous extraction significantly increases the extractable EtG content compared with cut hair. The effect ranged between 137 and 230% and was seen to occur regardless of the extent of pulverization. Cooling of samples was not necessary to prevent partial degradation of EtG during the grinding procedure. Conclusion: The optionsAim: The objective of the study was to provide conclusive evidence for the effect of particle size reduction as by milling on the extractable content of ethyl glucuronide (EtG) of hair samples. Methods: A number of real case hair samples and two pooled hair materials with EtG contents in the range of 10–30 pg/mg were systematically compared with regard to the extraction yield of EtG after cutting to 2–3 mm length and pulverization with a ball mill. After the respective treatment the samples were submitted to aqueous extraction followed by quantification of EtG using HPLC-MS/MS. Results: It was unequivocally demonstrated that milling of hair samples prior to aqueous extraction significantly increases the extractable EtG content compared with cut hair. The effect ranged between 137 and 230% and was seen to occur regardless of the extent of pulverization. Cooling of samples was not necessary to prevent partial degradation of EtG during the grinding procedure. Conclusion: The options currently employed at choice in analytical practice (cutting or milling) were seen to significantly affect the extractable amount of EtG in hair. This is suspected to influence the degree of equivalence of quantification results obtained in different laboratories as well as their respective classification of a test subject's drinking behaviour on the basis of currently recommended cut-off values.zeige mehrzeige weniger

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Metadaten
Autoren/innen:Bettina Mönch, Roland Becker, Irene Nehls
Dokumenttyp:Zeitschriftenartikel
Veröffentlichungsform:Verlagsliteratur
Sprache:Englisch
Titel des übergeordneten Werkes (Englisch):Alcohol and alcoholism
Jahr der Erstveröffentlichung:2013
Organisationseinheit der BAM:1 Analytische Chemie; Referenzmaterialien
1 Analytische Chemie; Referenzmaterialien / 1.7 Organische Spuren- und Lebensmittelanalytik
Herausgeber (Institution):Medical Council on Alcoholism
Verlag:Univ. Press
Verlagsort:Oxford
Jahrgang/Band:48
Ausgabe/Heft:5
Erste Seite:558
Letzte Seite:563
DOI:https://doi.org/10.1093/alcalc/agt059
ISSN:0735-0414
ISSN:0309-1635
ISSN:1464-3502
Verfügbarkeit des Volltexts:Volltext-PDF im Netzwerk der BAM verfügbar ("Closed Access")
Datum der Freischaltung:20.02.2016
Referierte Publikation:Ja
Datum der Eintragung als referierte Publikation:30.07.2013