Quantification of Three-Dimensional Spindle Architecture

Please always quote using this URN: urn:nbn:de:0297-zib-66562
  • Mitotic and meiotic spindles are microtubule-based structures to faithfully segregate chromosomes. Electron tomography is currently the method of choice to analyze the three-dimensional architecture of both types of spindles. Over the years, we have developed methods and software for automatic segmentation and stitching of microtubules in serial sections for large-scale reconstructions. Three-dimensional reconstruction of microtubules, however, is only the first step towards biological insight. The second step is the analysis of the structural data to derive measurable spindle properties. Here, we present a comprehensive set of techniques to quantify spindle parameters. These techniques provide quantitative analyses of specific microtubule classes and are applicable to a variety of tomographic reconstructions of spindles from different organisms.

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Author:Norbert Lindow, Stefanie Redemann, Gunar Fabig, Thomas Müller-Reichert, Steffen Prohaska
Document Type:ZIB-Report
Date of first Publication:2018/02/01
Series (Serial Number):ZIB-Report (18-07)
ISSN:1438-0064