Transmitted light pH optode for small sample volumes
- An innovative concept of a low-cost pH optode with working volumes of less than 150 µL is presented. The pH monitoring is based on the color changing effect of pH indicators. The optode includes an RGB color sensor patch TCS34725 from Adafruit, a controllable LED and reactor slides and is addressed by a self-written LabVIEW© software. Utilizing the hue value of the HSV color model, it is possible to analyze the color change of the indicator and estimate the pH value of the analyzed samples by exploiting sigmoidal fit models. Measurements carried out with phenol red and DMEM (Dulbecco's Modified Eagle's Medium) reported a standard error of calibration in the physiologic pH range (6.5–7.5) of ±0.04 pH units.
Author: | Christian Rogge, Steffen Zinn, Paolo Prosposito, Roberto Francini, Andreas Foitzik |
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URN: | urn:nbn:de:kobv:526-opus4-10093 |
DOI: | https://doi.org/10.5194/jsss-6-351-2017 |
ISSN: | 2194-878X |
Parent Title (English): | Journal of Sensors and Sensor Systems |
Document Type: | Article |
Language: | English |
Year of Publication: | 2017 |
Publishing Institution: | Technische Hochschule Wildau |
Release Date: | 2017/11/02 |
Volume: | 6 |
Issue: | 2 |
First Page: | 351 |
Last Page: | 359 |
Source: | Rogge, C., Zinn, S., Prosposito, P., Francini, R., and Foitzik, A. H.: Transmitted light pH optode for small sample volumes, J. Sens. Sens. Syst., 6, 351-359, https://doi.org/10.5194/jsss-6-351-2017, 2017. |
Faculties an central facilities: | Fachbereich Ingenieur- und Naturwissenschaften |
Dewey Decimal Classification: | 6 Technik, Medizin, angewandte Wissenschaften / 62 Ingenieurwissenschaften / 621 Angewandte Physik |
Licence (German): | Creative Commons - CC BY 3.0 - Namensnennung 3.0 Unported |