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The use of fluorescence microscopy and image analysis for rapid detection of non-producing revertant cells of Synechocystis sp. PCC6803 and Synechococcus sp. PCC7002

  • Ethanol production via genetically engineered cyanobacteria is a promising solution for the production of biofuels. Through the introduction of a pyruvate decarboxylase and alcohol dehydrogenase direct ethanol production becomes possible within the cells. However, during cultivation genetic instability can lead to mutations and thus loss of ethanol production. Cells then revert back to the wild type phenotype. A method for a rapid and simple detection of these non-producing revertant cells in an ethanol producing cell population is an important quality control measure in order to predict genetic stability and the longevity of a producing culture. Several comparable cultivation experiments revealed a difference in the pigmentation for non-producing and producing cells: the accessory pigment phycocyanin (PC) is reduced in case of the ethanol producer, resulting in a yellowish appearance of the culture. Microarray and western blot studies of Synechocystis sp. PCC6803 and Synechococcus sp. PCC7002 confirmed this PC reduction on the level of RNA and protein.

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Metadaten
Author:Katja Schulze, Imke Lang, Heike Enke, Diana Grohme, Marcus FrohmeORCiDGND
URN:urn:nbn:de:kobv:526-opus4-5911
DOI:https://doi.org/10.1186/s13104-015-1112-1
ISSN:1756-0500
Parent Title (English):BMC Research Notes
Document Type:Article
Language:English
Year of Publication:2015
Date of first Publication:2015/04/17
Publishing Institution:Technische Hochschule Wildau
Release Date:2016/06/28
Tag:3D fluorescence scan; PCC6803; PCC7002; absorption spectra; ethanol producer; fluorescence microscopy; genetic instability; image analysis; phycocyanin
Volume:8
Issue:160
Page Number:11
Source:Katja Schulze, Imke Lang, Heike Enke, Diana Grohme and Marcus Frohme. The use of fluorescence microscopy and image analysis for rapid detection of non-producing revertant cells of Synechocystis sp. PCC6803 and Synechococcus sp. PCC7002. BMC Research Notes 2015 8:160. DOI: 10.1186/s13104-015-1112-1. © Schulze et al.; licensee BioMed Central. 2015
Faculties an central facilities:Fachbereich Ingenieur- und Naturwissenschaften
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie
Licence (German):Creative Commons - CC BY - Namensnennung 4.0 International
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