A moonlighting role for enzymes of glycolysis in the co-localization of mitochondria and chloroplasts
- Glycolysis is one of the primordial pathways of metabolism, playing a pivotal role in energy metabolism and biosynthesis. Glycolytic enzymes are known to form transient multi-enzyme assemblies. Here we examine the wider protein-protein interactions of plant glycolytic enzymes and reveal a moonlighting role for specific glycolytic enzymes in mediating the co-localization of mitochondria and chloroplasts. Knockout mutation of phosphoglycerate mutase or enolase resulted in a significantly reduced association of the two organelles. We provide evidence that phosphoglycerate mutase and enolase form a substrate-channelling metabolon which is part of a larger complex of proteins including pyruvate kinase. These results alongside a range of genetic complementation experiments are discussed in the context of our current understanding of chloroplast-mitochondrial interactions within photosynthetic eukaryotes.
Author: | Youjun Zhang, Arun Sampathkumar, Sandra Mae-Lin Kerber, Corné Swart, Carsten HilleORCiD, Kumar Seerangan, Alexander Graf, Lee Sweetlove, Alisdair R. Fernie |
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URN: | urn:nbn:de:kobv:526-opus4-14812 |
DOI (Version of Record): | https://doi.org/10.1038/s41467-020-18234-w |
ISSN: | 2041-1723 |
Parent Title (English): | Nature Communications |
Document Type: | Article |
Language: | English |
Year of Publication: | 2020 |
Publishing Institution: | Technische Hochschule Wildau |
Release Date: | 2021/09/03 |
Volume: | 11 |
First Page: | 4509 |
Source: | Zhang, Y., Sampathkumar, A., Kerber, S.ML. et al. A moonlighting role for enzymes of glycolysis in the co-localization of mitochondria and chloroplasts. Nat Commun 11, 4509 (2020). https://doi.org/10.1038/s41467-020-18234-w |
Faculties an central facilities: | Zentrale Einrichtungen |
Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
Licence (German): | Creative Commons - CC BY - Namensnennung 4.0 International |