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Autocrats utilize (nominally) democratic elections, to claim procedural legitimation. To secure their political survival in these elections, they have an extensive menu of manipulation at their disposal. These manipulations are not only addressed at contestation but also inclusiveness of the elections. Although autocrats formally claim universal suffrage, informal restrictions and practices are implemented. Analyzing elections held between 1970 and 2020 in electoral autocracies, I find empiric evidence for strategic adjustments of suffrage rights as a response to electoral contexts.
Anthracyclines like daunorubicin (DRN) and doxorubicin (DOX) play an undisputed key role in cancer treatment, but their chronic administration can cause severe side effects. For precise anthracycline analytical systems, aptamers are preferable recognition elements. Here, we describe the detailed characterisation of a single-stranded DNA aptamer DRN-10 and its truncated versions for DOX and DRN detection. Binding affinities were determined from surface plasmon resonance (SPR) and microscale thermophoresis (MST) and combined with conformational data from circular dichroism (CD). Both aptamers displayed similar nanomolar binding affinities to DRN and DOX, even though their rate constants differed as shown by SPR recordings. SPR kinetic data unravelled a two-state reaction model including a 1 : 1 binding and a subsequent conformational change of the binding complex. This model was supported by CD spectra. In addition, the dissociation constants determined with MST were always lower than that from SPR, and especially for the truncated aptamer they differed by two orders of magnitude. This most probably reflects the methodological difference, namely labelling for MST vs. immobilisation for SPR. From CD recordings, we suggested a specific G-quadruplex as structural basis for anthracycline binding. We concluded that the aptamer DRN-10 is a promising recognition element for anthracycline detection systems and further selected aptamers can be also characterised with the combined methodological approach presented here.
Glycolysis is one of the primordial pathways of metabolism, playing a pivotal role in energy metabolism and biosynthesis. Glycolytic enzymes are known to form transient multi-enzyme assemblies. Here we examine the wider protein-protein interactions of plant glycolytic enzymes and reveal a moonlighting role for specific glycolytic enzymes in mediating the co-localization of mitochondria and chloroplasts. Knockout mutation of phosphoglycerate mutase or enolase resulted in a significantly reduced association of the two organelles. We provide evidence that phosphoglycerate mutase and enolase form a substrate-channelling metabolon which is part of a larger complex of proteins including pyruvate kinase. These results alongside a range of genetic complementation experiments are discussed in the context of our current understanding of chloroplast-mitochondrial interactions within photosynthetic eukaryotes.
Protein interaction and protein imaging strongly benefit from the advancements in time-resolved and superresolution fluorescence microscopic techniques. However, the techniques were typically applied separately and ex vivo because of technical challenges and the absence of suitable fluorescent protein pairs. Here, we show correlative in vivo fluorescence lifetime imaging microscopy Förster resonance energy transfer (FLIM-FRET) and stimulated emission depletion (STED) microscopy to unravel protein mechanics and structure in living cells. We use magnetotactic bacteria as a model system where two proteins, MamJ and MamK, are used to assemble magnetic particles called magnetosomes. The filament polymerizes out of MamK and the magnetosomes are connected via the linker MamJ. Our system reveals that bacterial filamentous structures are more fragile than the connection of biomineralized particles to this filament. More importantly, we anticipate the technique to find wide applicability for the study and quantification of biological processes in living cells and at high resolution.
Erinnerung an die Zukunft
(2020)
The following article investigates assessments of the Pacific Ocean as the ›sea of the future‹, following a Mediterranean past and an Atlantic present, and standing in a stark contrast to the ›exotic‹, marginalised view of its islands and shores. It analyses three German-language texts from the era of the Second World War: Egon Erwin Kisch’s travelogue Secret China (China geheim, 1933), Vicki Baum’s Hotel Shanghai (1939), and Alfred Döblin’s short expository text The Golden Gate (Das Goldene Tor, 1946). Despite the many variations in their portrayals of wars and crises, migrations and acculturations, breakdowns and new beginnings around the Pacific Ocean, all three authors evaluate the challenge posed by the transpacific triangle evolving around China, Japan, and the US to Europe’s self-postulated role as a leading continent. In that vein, they are (re-)activating a specific lieu de mémoire of a Pacific future in world affairs, in hopes of heightening transcultural self-understanding and self-positioning in times of crisis.