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3D printed biosensor for continuous glucose measurement in cell cultures

  • A novel 3D-printed glucose sensor is presented for cell culture application. Glucose sensing was performed using a fluorescence resonance energy transfer (FRET)-based assay principle based on ConA and dextran. Both molecules are encapsulated in alginate microspheres and embedded in the UV-curable, stable hydrogel polyvinyl alcohol (PVA). The rheology of the formulation was adapted to obtain good properties for an extrusion-based printing process. The printed sensor structures were tested for their ability to detect glucose in vitro. A proportional increase in fluorescence intensity was observed in a concentration range of 0 - 2 g/L glucose. Tests with HEK cell cultures also showed good cell compatibility and excellent adhesion properties on plasma-treated Petri dishes. The printed sensors were able to detect the glucose decay associated with the metabolic activities of the fast-growing HEK cells in the cell culture medium over ten days. The proof-of-principle study shows that metabolic processes in cell cultures can be monitored with the new printed sensor using a standard fluorescence wide-field microscope.
Metadaten
Author:Christiane Thielemann, Nenad Krstić, Jens Jüttner, Lars Giegerich, Margot Mayer, Monika Knuth, Achim Müller
DOI:https://doi.org/https://doi.org/10.1016/j.stlm.2023.100111
Parent Title (English):Annals of 3D Printed Medicine
Document Type:Article
Language:English
Year of Completion:2023
Release Date:2023/04/03
Tag:Bioprinting, FRET, Enzyme-free glucose sensor, PVA hydrogel
GND Keyword:Biosensor; 3D-Druck; Zellkultur
Volume:2023
Issue:-
First Page:-
Last Page:-
Urheberrecht:1
Institutes:Fakultät Ingenieurwissenschaften / Labore / BIOMEMS Lab
research focus :Materials / Material Testing & Sensor Technology
Licence (German):Creative Commons - CC BY-NC-ND - Namensnennung - Nicht kommerziell - Keine Bearbeitungen 4.0 International
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