Filtern
Dokumenttyp
Sprache
- Englisch (2)
Referierte Publikation
- ja (2)
Schlagworte
- Combinatorial chemistry (1)
- Combinatorial polymer libraries (1)
- Conjugates (1)
- Drug (1)
- ESI MS (1)
- MALDI-TOF (1)
- Mass spectrometry (1)
- PEG (1)
- Polyethylene glycol (1)
- Precision polymer sequencing (1)
Organisationseinheit der BAM
The detailed characterization of poly(styrene)-b-poly(tetrahydrofuran) (pS-b-pTHF) multiblock copolymers (17800 g mol-1 ≤ Mn ≤ 46800 g mol-1) generated via urethane linkages is presented. The synthesis of the block copolymers is enabled via a mechanistic switch of the thiocarbonyl thio end group of a poly(styrene) to dihydroxyl terminated polymers that subsequently react with a diisocyanate terminated polytetrahydrofuran based prepolymer to form multiblock copolymer structures. The characterization of the multiblock copolymers and their substructures includes size exclusion chromatography (SEC), liquid chromatography at critical conditions (LCCC), nuclear magnetic resonance (NMR), and infrared (IR) spectroscopy as well as matrix-assisted laser desorption/ionization (MALDI) mass spectrometry. To obtain even further details of the polymer size and its composition, SEC with triple detection as well as newly developed SEC coupled online to IR spectroscopy was carried out. The quantification of the average block fractions via online SEC-IR (4161 mol % pTHF) is in very good agreement with the results obtained via NMR spectroscopy (3966 mol % pTHF).
Functional sequences of precision polymers based on thiolactone/Michael chemistry are identified from a large one-bead one-compound library. Single-bead readout by MALDI-TOF MS/MS identifies sequences that host m-THPC that is a second Generation photo-sensitizer drug. The corresponding Tla/Michael-PEG conjugates make m-THPC available in solution and drug payload as well as drug release kinetics can be fine-tuned by the precision segment.