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Hepcidin-25 concentrations measured by various methods differ considerably, complicating interpretation. Here, a previously identified plasma-based candidate secondary reference material (csRM) was modified into a serum-based two-leveled sRM. We validated its functionality to increase the equivalence between methods for international standardization. We applied technical procedures developed by the International Consortium for Harmonization of Clinical Laboratory Results. The sRM, consisting of lyophilized serum with cryolyoprotectant, appeared commutable among nine different measurement procedures using 16 native human serum samples in a first round robin (RR1). Harmonization potential of the sRM was simulated in RR1 and evaluated in practice in RR2 among 11 measurement procedures using three native human plasma samples. Comprehensive purity analysis of a candidate primary RM (cpRM) was performed by state-of-the-art procedures. The sRM was value assigned with an isotope dilution mass spectrometry-based candidate reference method calibrated using the certified pRM. The inter-assay CV without harmonization was 42.1% and 52.8% in RR1 and RR2, respectively. In RR1, simulation of harmonization with sRM resulted in an inter-assay CV of 11.0%, whereas in RR2 calibration with the material resulted in an inter-assay CV of 19.1%. Both the sRM and pRM passed international homogeneity criteria and showed long-term stability. We assigned values to the low (0.95 ± 0.11 nmol/L) and middle concentration (3.75 ± 0.17 nmol/L) calibrators of the sRM. Standardization of hepcidin is possible with our sRM, which value is assigned by a pRM. We propose the implementation of this material as an international calibrator for hepcidin-25.
Numerical simulators, such as finite element models, have become increasingly capable of predicting the behaviour of structures and components owing to more sophisticated underlying mathematical models and advanced computing power. A common challenge lies, however, in calibrating these models in terms of their unknown/uncertain parameters. When measurements exist, this can be achieved by comparing the model response against measured data. Besides uncertain model parameters, phenomena like damage can give rise to further uncertainties; in particular, quasi-brittle materials, like concrete, experience damage in a heterogeneous manner due to various imperfections, e.g. in geometry and boundary conditions. This hardens an accurate prediction of the damaged behaviour of real structures that comprise such materials.
In this study, which draws from a data-driven approach, we use the force-version of the finite element model updating method (FEMU-F) to incorporate measured displacements into the identification of the damage parameters, in order to cope with heterogeneity. In this method, instead of conducting a forward evaluation of the model and comparing the model response (displacements) against the data, we impose displacements to the model and compare the resulting force residuals with measured reaction forces. To account for uncertainties in the measurement of displacements, we endow this approach with a penalty term, which reflects the discrepancy between measured and imposed displacements, where the latter is assumed as unknown random variables to be identified as well. A Variational Bayesian approach is used as an approximating tool for computing posterior parameters. The underlying damage model considered in this work is a gradient-enhanced damage model.
We first establish the identification procedure through two virtual examples, where synthetic data (displacements) are generated over a certain spatially-dense set of points over the domain. The procedure is then validated on an experimental case-study; namely a 3-point bending experiment with displacement measurements resulting from a digital image correlation (DIC) analysis.
A controlled, reproducible, gram-scale method is reported for the covalent functionalization of graphene Sheets by a one-pot nitrene [2+1] cycloaddition reaction under mild conditions. The reaction between commercially available 2,4,6-trichloro-1,3,5-triazine and sodium azide with thermally reduced graphene oxide (TRGO) results in defined dichlorotriazine-functionalized sheets. The different reactivities of the chlorine substituents on the functionalized graphene allow stepwise post-modification by manipulating the temperature.
This new method provides unique access to defined bifunctional 2D nanomaterials, as exemplified by chiral surfaces and multifunctional hybrid architectures.