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To investigate the attachment behavior of bacteria, tailored surfaces are needed. Differences in the chemical charge of the substrate are varied by -COO-, -CH3, -OH, -NH3 terminated functional groups. Regular patterns of them on the surfaces enable bacteria to choose their preferred attachment sides. Tools to tailor the samples are µ-contact printing, self-assembled monolayers and physical vapor deposition. The same concept of preferred sides in patterned surfaces is transferred to the nano- and microroughness which is accomplished by using zinc oxide nanorods synthesis.