Spectroscopic characterization of plasma - Chemically functionalized and fluorophore-labeled polymer surfaces
- The potential of spectrofluorometry and fluorescence microscopy for the characterization and quantification of different functionalities like OH and NH2 groups at plasma-chemically modified polymer surfaces is assessed using traditional reactive dyes such as dansyl derivatives and a sophisticated VIS-excitable chromogenic and fluorogenic pyrylium label showing binding-induced spectral and intensity changes in absorption and emission. Aiming at an improved fluorometric surface analysis, based upon these measurements, several sources of uncertainty inherent to fluorescence measurements are illustrated ranging from environment-dependent dye absorption and emission features over spectral correction and nonspecific adsorption to the critical influence of label choice on the measured background. Solutions to these drawbacks are given thereby underlining the potential of fluorometry for surface analysis.
Autor*innen: | Katrin HoffmannORCiD, Renate Mix, Jörg Florian Friedrich, Ute Resch-GengerORCiD |
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Persönliche Herausgeber*innen: | C.D. Geddes |
Dokumenttyp: | Beitrag zu einem Sammelband |
Veröffentlichungsform: | Verlagsliteratur |
Sprache: | Englisch |
Titel des übergeordneten Werkes (Englisch): | Reviews in fluorescence 2008 |
Jahr der Erstveröffentlichung: | 2010 |
Verlag: | Springer Science + Business Media |
Jahrgang/Band: | 5 |
Erste Seite: | 139 |
Letzte Seite: | 160 |
Freie Schlagwörter: | Fluorescence spectroscopy; Plasma modification; Polymer functionalization; Surface labeling |
DOI: | 10.1007/978-1-4419-1260-2_6 |
ISBN: | 978-1-4419-0828-5 |
Bemerkung: | Serientitel: Reviews in Fluorescence – Series title: Reviews in Fluorescence |
Verfügbarkeit des Dokuments: | Physisches Exemplar in der Bibliothek der BAM vorhanden ("Hardcopy Access") |
Bibliotheksstandort: | Sonderstandort: Publica-Schrank |
Datum der Freischaltung: | 19.02.2016 |
Referierte Publikation: | Nein |