Comparability of fluorescence microscopy data and need for instrument characterization of spectral scanning microscopes
- The aim of this article is to illustrate the need for an improved quality assurance in fluorescence microscopy. From the instrument-side, this can be achieved by a better understanding, consideration, and regular control of the instrument-specific parameters and quantities affecting measured fluorescence signals. Particularly, the need for requirements on physical- and chemical-type instrument standards for the characterization and performance validation of spectral fluorescence microscopes (SFMs) is discussed and suitable systems are presented. Special emphasis is given to spectral fluorescence standards and to day-to-day intensity standards for SFMs. Fluorescence standards and well-characterized fluorescence microscopes are the first and essential steps towards the comparability and the understanding of the variability in fluorescence microscopy data in medical and life sciences. In addition, standards enable the distinction between instrument-specific variations and fluorescentThe aim of this article is to illustrate the need for an improved quality assurance in fluorescence microscopy. From the instrument-side, this can be achieved by a better understanding, consideration, and regular control of the instrument-specific parameters and quantities affecting measured fluorescence signals. Particularly, the need for requirements on physical- and chemical-type instrument standards for the characterization and performance validation of spectral fluorescence microscopes (SFMs) is discussed and suitable systems are presented. Special emphasis is given to spectral fluorescence standards and to day-to-day intensity standards for SFMs. Fluorescence standards and well-characterized fluorescence microscopes are the first and essential steps towards the comparability and the understanding of the variability in fluorescence microscopy data in medical and life sciences. In addition, standards enable the distinction between instrument-specific variations and fluorescent label- or probe-related uncertainties as well as generally sample-related effects.…
Autor*innen: | Katrin HoffmannORCiD, Ute Resch-GengerORCiD, R. Nitschke |
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Persönliche Herausgeber*innen: | O.S. Wolfbeis, Ute Resch-Genger |
Dokumenttyp: | Beitrag zu einem Sammelband |
Veröffentlichungsform: | Verlagsliteratur |
Sprache: | Englisch |
Titel des übergeordneten Werkes (Englisch): | Standardization and quality assurance in fluorescence measurements II - Bioanalytical and biomedical applications |
Jahr der Erstveröffentlichung: | 2008 |
Verlag: | Springer |
Verlagsort: | Berlin, Heidelberg |
Jahrgang/Band: | 6 |
Ausgabe/Heft: | Part A |
Erste Seite: | 89 |
Letzte Seite: | 116 |
Freie Schlagwörter: | Calibration; Comparability; Fluorescence microscopy; Fluorescence spectroscopy; Quality assurance; Quantification; Standard |
DOI: | 10.1007/4243_2008_028 |
ISSN: | 1617-1306 |
ISBN: | 978-3-540-70570-3 |
Bemerkung: | Serientitel: Springer Series on Fluorescence – Series title: Springer Series on Fluorescence |
Verfügbarkeit des Dokuments: | Physisches Exemplar in der Bibliothek der BAM vorhanden ("Hardcopy Access") |
Bibliotheksstandort: | Sonderstandort: Publica-Schrank |
Datum der Freischaltung: | 19.02.2016 |
Referierte Publikation: | Nein |