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Comparability of fluorescence microscopy data and need for instrument characterization of spectral scanning microscopes

  • The aim of this article is to illustrate the need for an improved quality assurance in fluorescence microscopy. From the instrument-side, this can be achieved by a better understanding, consideration, and regular control of the instrument-specific parameters and quantities affecting measured fluorescence signals. Particularly, the need for requirements on physical- and chemical-type instrument standards for the characterization and performance validation of spectral fluorescence microscopes (SFMs) is discussed and suitable systems are presented. Special emphasis is given to spectral fluorescence standards and to day-to-day intensity standards for SFMs. Fluorescence standards and well-characterized fluorescence microscopes are the first and essential steps towards the comparability and the understanding of the variability in fluorescence microscopy data in medical and life sciences. In addition, standards enable the distinction between instrument-specific variations and fluorescentThe aim of this article is to illustrate the need for an improved quality assurance in fluorescence microscopy. From the instrument-side, this can be achieved by a better understanding, consideration, and regular control of the instrument-specific parameters and quantities affecting measured fluorescence signals. Particularly, the need for requirements on physical- and chemical-type instrument standards for the characterization and performance validation of spectral fluorescence microscopes (SFMs) is discussed and suitable systems are presented. Special emphasis is given to spectral fluorescence standards and to day-to-day intensity standards for SFMs. Fluorescence standards and well-characterized fluorescence microscopes are the first and essential steps towards the comparability and the understanding of the variability in fluorescence microscopy data in medical and life sciences. In addition, standards enable the distinction between instrument-specific variations and fluorescent label- or probe-related uncertainties as well as generally sample-related effects.zeige mehrzeige weniger

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Metadaten
Autor*innen:Katrin HoffmannORCiD, Ute Resch-GengerORCiD, R. Nitschke
Persönliche Herausgeber*innen: O.S. Wolfbeis, Ute Resch-Genger
Dokumenttyp:Beitrag zu einem Sammelband
Veröffentlichungsform:Verlagsliteratur
Sprache:Englisch
Titel des übergeordneten Werkes (Englisch):Standardization and quality assurance in fluorescence measurements II - Bioanalytical and biomedical applications
Jahr der Erstveröffentlichung:2008
Verlag:Springer
Verlagsort:Berlin, Heidelberg
Jahrgang/Band:6
Ausgabe/Heft:Part A
Erste Seite:89
Letzte Seite:116
Freie Schlagwörter:Calibration; Comparability; Fluorescence microscopy; Fluorescence spectroscopy; Quality assurance; Quantification; Standard
DOI:10.1007/4243_2008_028
ISSN:1617-1306
ISBN:978-3-540-70570-3
Bemerkung:
Serientitel: Springer Series on Fluorescence – Series title: Springer Series on Fluorescence
Verfügbarkeit des Dokuments:Physisches Exemplar in der Bibliothek der BAM vorhanden ("Hardcopy Access")
Bibliotheksstandort:Sonderstandort: Publica-Schrank
Datum der Freischaltung:19.02.2016
Referierte Publikation:Nein
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