Insights into Interprotein Electron Transfer of Human Cytochrome c Variants Arranged in Multilayer Architectures by Means of an Artificial Silica Nanoparticle Matrix

  • The redox behavior of proteins plays a crucial part in the design of bioelectronic systems. We have demonstrated several functional systems exploiting the electron exchange properties of the redox protein cytochrome c (cyt c) in combination with enzymes and photoactive proteins. The operation is based on an effective reaction at modified electrodes but also to a large extent on the capability of self-exchange between cyt c molecules in a surface-fixed state. In this context, different variants of human cyt c have been examined here with respect to an altered heterogeneous electron transfer (ET) rate in a monolayer on electrodes as well as an enhanced self-exchange rate while being incorporated in multilayer architectures. For this purpose, mutants of the wild-type (WT) protein have been prepared to change the chemical nature of the surface contact area near the heme edge. The structural integrity of the variants has been verified by NMR and UV–vis measurements. It is shown that the single-point mutations can significantly influence the heterogeneous ET rate at thiol-modified gold electrodes and that electroactive protein/silica nanoparticle multilayers can be constructed with all forms of human cyt c prepared. The kinetic behavior of electron exchange for the mutant proteins in comparison with that of the WT has been found altered in some multilayer arrangements. Higher self-exchange rates have been found for K79A. The results demonstrate that the position of the introduced change in the charge situation of cyt c has a profound influence on the exchange behavior. In addition, the behavior of the cyt c variants in assembled multilayers is found to be rather similar to the situation of cyt c self-exchange in solution verified by NMR.

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Metadaten
Author:Sven Christian Feifel, Kai Ralf Stieger, Andreas Kapp, Dennis Weber, Marco Allegrozzi, Mario Piccioli, Paola Turano, Fred LisdatORCiDGND
URN:urn:nbn:de:kobv:526-opus4-6753
DOI:https://doi.org/10.1021/acsomega.6b00213
ISSN:2470-1343
Parent Title (English):ACS Omega
Document Type:Article
Language:English
Year of Publication:2016
Publishing Institution:Technische Hochschule Wildau
Release Date:2016/12/14
Volume:1
Issue:6
First Page:1058
Last Page:1066
Source:Sven Christian Feifel, Kai Ralf Stieger, Andreas Kapp, Dennis Weber, Marco Allegrozzi, Mario Piccioli, Paola Turano, and Fred Lisdat: Insights into Interprotein Electron Transfer of Human Cytochrome c Variants Arranged in Multilayer Architectures by Means of an Artificial Silica Nanoparticle Matrix. ACS Omega 2016 1 (6), 1058-1066. DOI: 10.1021/acsomega.6b00213
Faculties an central facilities:Fachbereich Ingenieur- und Naturwissenschaften
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 54 Chemie / 540 Chemie und zugeordnete Wissenschaften
Licence (English):ACS AuthorChoice/Editors’ Choice
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