Filtern
Erscheinungsjahr
Dokumenttyp
- Zeitschriftenartikel (15)
- Beitrag zu einem Tagungsband (2)
- Buchkapitel (1)
Schlagworte
Organisationseinheit der BAM
- 9 Komponentensicherheit (7)
- 9.3 Schweißtechnische Fertigungsverfahren (6)
- 6 Materialchemie (2)
- 6.2 Material- und Oberflächentechnologien (2)
- 1 Analytische Chemie; Referenzmaterialien (1)
- 1.2 Biophotonik (1)
- 4 Material und Umwelt (1)
- 4.1 Biologische Materialschädigung und Referenzorganismen (1)
- 6.1 Oberflächen- und Dünnschichtanalyse (1)
- 9.5 Tribologie und Verschleißschutz (1)
Silver nanoparticles (SNP) are the subject of worldwide commercialization because of their antimicrobial effects. Yet only little data on their mode of action exist. Further, only few techniques allow for visualization and quantification of unlabeled nanoparticles inside cells. To study SNP of different sizes and coatings within human macrophages, we introduce a novel laser postionization secondary neutral mass spectrometry (Laser-SNMS) approach and prove this method superior to the widely applied confocal Raman and transmission electron microscopy. With time-of-flight secondary ion mass spectrometry (TOF-SIMS) we further demonstrate characteristic fingerprints in the lipid pattern of the cellular membrane indicative of oxidative stress and membrane fluidity changes. Increases of protein carbonyl and heme oxygenase-1 levels in treated cells confirm the presence of oxidative stress biochemically. Intriguingly, affected phagocytosis reveals as highly sensitive end point of SNP-mediated adversity in macrophages. The cellular responses monitored are hierarchically linked, but follow individual kinetics and are partially reversible.
Silver nanoparticles (SNP) are among the most commercialized nanoparticles worldwide. They can be found in many diverse products, mostly because of their antibacterial properties. Despite its widespread use only little data on possible adverse health effects exist. It is difficult to compare biological data from different studies due to the great variety in sizes, coatings or shapes of the particles. Here, we applied a novel synthesis approach to obtain SNP, which are covalently stabilized by a small peptide. This enables a tight control of both size and shape. We applied these SNP in two different sizes of 20 or 40 nm (Ag20Pep and Ag40Pep) and analyzed responses of THP-1-derived human macrophages. Similar gold nanoparticles with the same coating (Au20Pep) were used for comparison and found to be non-toxic. We assessed the cytotoxicity of particles and confirmed their cellular uptake via transmission electron microscopy and confocal Raman microscopy. Importantly a majority of the SNP could be detected as individual particles spread throughout the cells. Furthermore we studied several types of oxidative stress related responses such as induction of heme oxygenase I or formation of protein carbonyls. In summary, our data demonstrate that even low doses of SNP exerted adverse effects in human macrophages.
Im Rahmen eines AiF-Forschungsvorhabens wurde die Beeinflussung des Schweißprozesses mittels generierter Wechselstrom-Magnetfelder (ACMagnetfelder) beim Laserstrahlschweißen von Aluminiumlegierungen untersucht. Das vorrangige Augenmerk galt hierbei der Entfernung von Poren sowie der Stabilisierung der Schweißnahtoberfläche zur Vermeidung von rauen Schweißnähten. Das Schweißen mit Einsatz des AC-Magneten erzeugte im Vergleich zu den Referenznähten deutlich flachere Schweißnähte mit reduzierter Porenanzahl, die typische Schuppenstruktur wurde unterdrückt. Das Magnetfeld bewirkte bei entsprechenden Parametern der magnetischen Flussdichte und der Frequenz eine Halbierung der Rauigkeit der Schweißnahtoberfläche. Neben der Oberflächenberuhigung wurde auch die Verteilung von Poren in der Schmelze beeinflusst. Es konnte nachgewiesen werden, dass bei einer geeigneten Auswahl der Parameter von AC-Magnetfeldern die Porositätsanteile in der Schweißnaht auf ein Zehntel gegenüber den Referenzschweißnähten reduziert werden können.-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
Within the framework of an AiF research project, investigations were conducted into the influences exerted on the welding process using generated alternating current (AC) magnetic fields during the laser beam welding of aluminium alloys. In this respect, attention was principally paid to removing pores and to stabilising the weld surface in order to avoid rough welds. In comparison with the reference welds, welding utilising the AC magnet produced considerably flatter welds with a reduced number of pores and the typical ripple structure was suppressed. With corresponding parameters for the magnetic flux density and the frequency, the roughness of the weld surface was halved using the magnetic field. In addition to the surface stabilisation, influences were also exerted on the distribution of pores in the molten metal. It was possible to prove that, by selecting suitable parameters for AC magnetic fields, the porosity proportions in the weld can be reduced to one tenth of those in the reference welds.
Maintenance, Repair and Overhaul (MRO) is acquiring increasing commercial and socio-economic significance. For products and goods with high investment costs and a long lifespan, especially in the sectors of energy and transportation, a considerable portion of commercial profits are generated by after-sales services. In the field of research and development, not enough attention has been paid so far to tasks and approaches involving MRO. The field thus has a limited scientific background, despite a high potential in the business sector for technological and scientific optimization. The challenges and chances of MRO for sustainable enterprises will be explained with reference to the Fraunhofer Innovation Cluster Maintenance, Repair and Overhaul in Energy and Transport. The developments and project results of the four fields of innovation »Cleaning«, »Repair and Overhaul«, »Condition Monitoring and Diagnosis«, as well as »MRO Planning and Digital Assistance« will be explained.
Among the various welding technologies, resistance spot welding (RSW) and laser beam welding (LBW) play a significant role as joining methods for the automobile industry. The application of RSW and LBW for the automotive body alters the microstructure in the welded areas. It is necessary to identify the mechanical properties of the welded material to be able to make a reliable statement about the material behavior and the strength of welded components. This study develops a method by which to determine the mechanical properties for the weldment of RSW and LBW for two dual phase (DP) steels, DP600 and DP1000, which are commonly used for the automotive bodies. The mechanical properties of the resistance spot weldment were obtained by performing tensile tests on the notched tensile specimen to cause an elongation of the notched and welded area in order to investigate its properties. In order to determine the mechanical properties of the laser beam weldment, indentation tests were performed on the welded material to calculate its force-penetration depth-curve. Inverse numerical simulation was used to simulate the indentation tests to determine and verify the parameters of a nonlinear isotropic material model for the weldment of LBW. Furthermore, using this method, the parameters for the material model of RSW were verified. The material parameters and microstructure of the weldment of RSW and LBW are compared and discussed. The results show that the novel method introduced in this work is a valid approach to determine the mechanical properties of welded high-strength steel structures. In addition, it can be seen that LBW and RSW lead to a reduction in ductility and an increase in the amount of yield and tensile strength of both DP600 and DP1000.
The impact of femtosecond (fs) laser-induced periodic surface structures (LIPSS) on tribological properties was investigated for metal-reinforced ceramic composites (Al2O3-ZrO2-Nb). For this purpose, the metallic niobium (Nb) phase was selectively structured with LIPSS in an air environment with different values of the fs-laser peak fluence by near-infrared fs-laser radiation (λ = 1025 nm, τ = 300 fs, frep = 1 kHz), taking advantage of the different light absorption behavior of ceramic and metal. The tribological performance was evaluated by reciprocating sliding tests in a ball-on-disc configuration using Ringer's solution as lubricant. The surfaces were characterized before and after laser irradiation by optical microscopy, scanning electron microscopy, atomic force microscopy, X-ray photoelectron spectroscopy, energy dispersive X-ray spectroscopy and by measuring the contact angle with Ringer's solution. The LIPSS formation resulted in an increased wetting of the surface with the lubricant. Moreover, the selectively structured composite surfaces revealed a coefficient of friction significantly reduced by a factor of ~3 when compared to the non-irradiated surface. Furthermore, the formation of a laser-induced oxidation layer was detected with NbO as the most prominent oxidation state. Selectively structured composites with outstanding mechanical properties and enhanced tribological performance are of particular interest for biomedical applications.
In additive manufacturing (AM) Laser Metal Deposition (LMD), parts are built by welding layers of powder feedstock onto a substrate. Applications for steel powders include forging tools and structural components for various industries. For large parts, the choice of tool-paths influences the build-rate, the part performance and the distortions in a highly geometry-dependent manner. With weld-path lengths in the range of hundreds of meters, a reliable, automated tool path generation is essential for the usability of LMD processes.
In this contribution, automated tool-path generation approaches are shown and their results are discussed for arbitrary geometries. The investigated path strategies are the classical approaches: “Zig-zag-” and “contour-parallel-strategies”. After generation, the tool-paths are automatically formatted into g-code for experimental build-up and ASCII for a numerical simulation model. Finally, the tool paths are discussed in regards to volume-fill, microstructure and porosity for the experimental samples.
This work presents a part of the IGF project 18737N “Welding distortion simulation” (FOSTA P1140)
This paper demonstrates that the instrumented indentation test (IIT), together with a trained artificial neural network (ANN), has the capability to characterize the mechanical properties of the local parts of a welded steel structure such as a weld nugget or heat affected zone.
Aside from force-indentation depth curves generated from the IIT, the profile of the indented surface deformed after the indentation test also has a strong correlation with the materials’ plastic behavior. The profile of the indented surface was used as the training dataset to design an ANN to determine the material parameters of the welded zones. The deformation of the indented surface in three dimensions shown in images were analyzed with the computer vision algorithms and the obtained data were employed to train the ANN for the characterization of the mechanical properties. Moreover, this method was applied to the images taken with a simple light microscope from the surface of a specimen. Therefore, it is possible to quantify the mechanical properties of the automotive steels with the four independent methods: (1) force-indentation depth curve; (2) profile of the indented surface; (3) analyzing of the 3D-measurement image; and (4) evaluation of the images taken by a simple light microscope. The results show that there is a very good Agreement between the material parameters obtained from the trained ANN and the experimental uniaxial tensile test. The results present that the mechanical properties of an unknown steel can be determined by only analyzing the images taken from its surface after pushing a simple indenter into its surface.
Additive manufacturing is no longer just used for the production of prototypes but already found its way into the industrial production. However, the fabrication of massive metallic parts with high geometrical complexity is still too time-consuming to be economically viable. The combination of the powder bed-based selective laser melting process (SLM), known for its geometrical freedom and accuracy, and the nozzle-based laser metal deposition process (LMD), known for its high build-up rates, has great potential to reduce the process duration. For the industrial application of the SLM-LMD hybrid process chain it is necessary to investigate the interaction of the processes and its effect on the material properties to guarantee part quality and prevent component failure. Therefore, hybrid components are manufactured and examined before and after the heat treatment regarding the microstructure and the hardness in the SLM-LMD transition zone. The experiments are conducted using the nickel-based alloy Inconel 718.
The combination of laser powder bed fusion (LPBF), known for its geometrical freedom and accuracy, and the nozzle-based laser metal deposition process (LMD), known for its high build-up rates, has great potential to reduce the additive manufacturing times for large metallic parts. For the industrial application of the LPBF-LMD hybrid process chain, it is necessary to investigate the infuence of the LMD process on the LPBF substrate. In addition, the build plate material also has a signifcant impact on the occurrence of distortion along the additive manufacturing process chain. In the literature, steel build plates are often used in laser-based additive manufacturing processes of Inconel 718, since a good metallurgical Bonding can be assured whilst reducing costs in the production and restoration of the build plates. This paper examines the distortion caused by LMD material deposition and the infuence of the build plate material along the hybrid additive manufacturing process chain. Twin cantilevers are manufactured by LPBF and an additional layer is subsequently deposited with LMD. The distortion is measured in the as-built condition as well as after heat treatment. The efect of diferent LMD hatch strategies on the distortion is determined. The experiments are conducted using the nickel-base alloy Inconel 718. The results show a signifcant infuence of LMD path strategies on distortion, with shorter tool paths leading to less distortion. The remaining distortion after heat treatment is considerably dependent on the material of the build plate.