Quantitative spectroscopy of single molecule interaction times

  • Single molecule fluorescence tracking provides information at nm-scale and ms-temporal resolution about the dynamics and interaction of individual molecules in a biological environment. While the dynamic behavior of isolated molecules can be characterized well, the quantitative insight is more limited when interactions between two indistinguishable molecules occur. We address here this aspect by developing a theoretical framework for a spectroscopy of interaction times. The non trivial crossover between a power law to an exponential behavior of the distribution of the interaction times is highlighted here, together with the dependence of the exponential term upon the product of the microscopic reaction rates (affinity). Our approach is validated on simulated as well as experimental datasets.

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Author:Horst-Holger Boltz, Alexei Sirbu, Nina Stelzer, Martin J. Lohse, Christof Schütte, Paolo Annible
Document Type:Article
Year of first publication:2020