Modeling of Multivalent Ligand-Receptor Binding Measured by kinITC

  • In addition to the conventional Isothermal Titration Calorimetry (ITC), kinetic ITC (kinITC) not only gains thermodynamic information, but also kinetic data from a biochemical binding process. Moreover, kinITC gives insights into reactions consisting of two separate kinetic steps, such as protein folding or sequential binding processes. The ITC method alone cannot deliver kinetic parameters, especially not for multivalent bindings. This paper describes how to solve the problem using kinITC and an invariant subspace projection. The algorithm is tested for multivalent systems with different valencies.
Metadaten
Author:Franziska Erlekam, Sinaida Igde, Susanna RöblitzORCiD, Laura Hartmann, Marcus Weber
Document Type:Article
Parent Title (English):Computation
Volume:7
Issue:3
First Page:46
Year of first publication:2019
DOI:https://doi.org/10.3390/computation7030046