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Quantification of three-dimensional spindle architecture

  • Mitotic and meiotic spindles are microtubule-based structures to faithfully segregate chromosomes. Electron tomography is currently the method of choice to analyze the three-dimensional (3D) architecture of both types of spindles. Over the years, we have developed methods and software for automatic segmentation and stitching of microtubules in serial sections for large-scale reconstructions. 3D reconstruction of microtubules, however, is only the first step toward biological insight. The second step is the analysis of the structural data to derive measurable spindle properties. Here, we present a comprehensive set of techniques to quantify spindle parameters. These techniques provide quantitative analyses of specific microtubule classes and are applicable to a variety of tomographic reconstructions of spindles from different organisms.
Author:Norbert LindowORCiD, Stefanie Redemann, Florian Brünig, Gunar FabigORCiD, Thomas Müller-Reichert, Steffen Prohaska
Document Type:Book chapter
Parent Title (English):Methods in Cell Biology Part B
First Page:45
Last Page:64
Publisher:Academic Press
Year of first publication:2018
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