TY - GEN A1 - Özel, M. Neset A1 - Kulkarni, Abhishek A1 - Hasan, Amr A1 - Brummer, Josephine A1 - Moldenhauer, Marian A1 - Daumann, Ilsa-Maria A1 - Wolfenberg, Heike A1 - Dercksen, Vincent J. A1 - Kiral, F. Ridvan A1 - Weiser, Martin A1 - Prohaska, Steffen A1 - von Kleist, Max A1 - Hiesinger, Peter Robin T1 - Serial synapse formation through filopodial competition for synaptic seeding factors N2 - Following axon pathfinding, growth cones transition from stochastic filopodial exploration to the formation of a limited number of synapses. How the interplay of filopodia and synapse assembly ensures robust connectivity in the brain has remained a challenging problem. Here, we developed a new 4D analysis method for filopodial dynamics and a data-driven computational model of synapse formation for R7 photoreceptor axons in developing Drosophila brains. Our live data support a 'serial synapse formation' model, where at any time point only a single 'synaptogenic' filopodium suppresses the synaptic competence of other filopodia through competition for synaptic seeding factors. Loss of the synaptic seeding factors Syd-1 and Liprin-α leads to a loss of this suppression, filopodial destabilization and reduced synapse formation, which is sufficient to cause the destabilization of entire axon terminals. Our model provides a filopodial 'winner-takes-all' mechanism that ensures the formation of an appropriate number of synapses. T3 - ZIB-Report - 19-45 KW - filopodia KW - growth cone dynamics KW - brain wiring KW - 2-photon microscopy KW - model Y1 - 2019 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:0297-zib-74397 SN - 1438-0064 ER - TY - CHAP A1 - Pfister, Hanspeter A1 - Kaynig, Verena A1 - Botha, Charl P. A1 - Bruckner, Stefan A1 - Dercksen, Vincent J. A1 - Hege, Hans-Christian A1 - Roerdink, Jos B.T.M. ED - Hansen, Charles D. ED - Chen, Min ED - Johnson, Christopher R. ED - Kaufman, Arie E. ED - Hagen, Hans T1 - Visualization in Connectomics T2 - Scientific Visualization - Uncertainty, Multifield, Biomedical, and Scalable Visualization N2 - Connectomics is a branch of neuroscience that attempts to create a connectome, i.e., a complete map of the neuronal system and all connections between neuronal structures. This representation can be used to understand how functional brain states emerge from their underlying anatomical structures and how dysfunction and neuronal diseases arise. We review the current state-of-the-art of visualization and image processing techniques in the field of connectomics and describe a number of challenges. After a brief summary of the biological background and an overview of relevant imaging modalities, we review current techniques to extract connectivity information from image data at macro-, meso- and microscales. We also discuss data integration and neural network modeling, as well as the visualization, analysis and comparison of brain networks. Y1 - 2014 SN - 978-1-4471-6496-8 U6 - https://doi.org/10.1007/978-1-4471-6497-5_21 SP - 221 EP - 245 PB - Springer ER - TY - GEN A1 - Pfister, Hanspeter A1 - Kaynig, Verena A1 - Botha, Charl A1 - Bruckner, Stefan A1 - Dercksen, Vincent J. A1 - Hege, Hans-Christian A1 - Roerdink, Jos T1 - Visualization in Connectomics Y1 - 2012 U6 - https://doi.org/10.1007/978-1-4471-6497-5_21 ER - TY - CHAP A1 - Papazov, Chavdar A1 - Dercksen, Vincent J. A1 - Lamecker, Hans A1 - Hege, Hans-Christian T1 - Visualizing morphogenesis and growth by temporal interpolation of surface-based 3D atlases T2 - Proceedings of the 2008 IEEE International Symposium on Biomedical Imaging Y1 - 2008 U6 - https://doi.org/10.1109/ISBI.2008.4541123 SP - 824 EP - 827 ER - TY - JOUR A1 - Ozel, Mehmet Neset A1 - Kulkarni, Abhishek A1 - Hasan, Amr A1 - Brummer, Josephine A1 - Moldenhauer, Marian A1 - Daumann, Ilsa-Maria A1 - Wolfenberg, Heike A1 - Dercksen, Vincent J. A1 - Kiral, Ferdi Ridvan A1 - Weiser, Martin A1 - Prohaska, Steffen A1 - von Kleist, Max A1 - Hiesinger, Peter Robin T1 - Serial synapse formation through filopodial competition for synaptic seeding factors JF - Developmental Cell N2 - Following axon pathfinding, growth cones transition from stochastic filopodial exploration to the formation of a limited number of synapses. How the interplay of filopodia and synapse assembly ensures robust connectivity in the brain has remained a challenging problem. Here, we developed a new 4D analysis method for filopodial dynamics and a data-driven computational model of synapse formation for R7 photoreceptor axons in developing Drosophila brains. Our live data support a 'serial synapse formation' model, where at any time point only a single 'synaptogenic' filopodium suppresses the synaptic competence of other filopodia through competition for synaptic seeding factors. Loss of the synaptic seeding factors Syd-1 and Liprin-α leads to a loss of this suppression, filopodial destabilization and reduced synapse formation, which is sufficient to cause the destabilization of entire axon terminals. Our model provides a filopodial 'winner-takes-all' mechanism that ensures the formation of an appropriate number of synapses. Y1 - 2019 U6 - https://doi.org/10.1016/j.devcel.2019.06.014 VL - 50 IS - 4 SP - 447 EP - 461 ER - TY - GEN A1 - Oberlaender, Marcel A1 - Dercksen, Vincent J. A1 - Lang, Stefan A1 - Sakmann, Bert T1 - 3D mapping of synaptic connections within “in silico” microcircuits of full compartmental neurons in extended networks on the example of VPM axons projecting into S1 of rats. T2 - Conference Abstract, Neuroinformatics Y1 - 2009 U6 - https://doi.org/10.3389/conf.neuro.11.2009.08.092 ER - TY - JOUR A1 - Oberlaender, Marcel A1 - Dercksen, Vincent J. A1 - Egger, Robert A1 - Gensel, Maria A1 - Sakmann, Bert A1 - Hege, Hans-Christian T1 - Automated three-dimensional detection and counting of neuron somata JF - Journal of Neuroscience Methods Y1 - 2009 U6 - https://doi.org/10.1016/j.jneumeth.2009.03.008 VL - 180 IS - 1 SP - 147 EP - 160 ER - TY - GEN A1 - Oberlaender, Marcel A1 - Dercksen, Vincent J. A1 - Broser, Philip J. A1 - Bruno, Randy M. A1 - Sakmann, Bert T1 - NeuroMorph and NeuroCount: Automated tools for fast and objective acquisition of neuronal morphology for quantitative structural analysis T2 - Frontiers in Neuroinformatics. Conference Abstract: Neuroinformatics Y1 - 2008 U6 - https://doi.org/10.3389/conf.neuro.11.2008.01.065 ER - TY - JOUR A1 - Oberlaender, Marcel A1 - de Kock, Christiaan P. J. A1 - Bruno, Randy M. A1 - Ramirez, Alejandro A1 - Meyer, Hanno A1 - Dercksen, Vincent J. A1 - Helmstaedter, Moritz A1 - Sakmann, Bert T1 - Cell Type-Specific Three-Dimensional Structure of Thalamocortical Circuits in a Column of Rat Vibrissal Cortex JF - Cerebral Cortex Y1 - 2012 U6 - https://doi.org/doi:10.1093/cercor/bhr317 VL - 22 IS - 10 SP - 2375 EP - 2391 ER - TY - GEN A1 - Oberlaender, Marcel A1 - Bruno, Randy M. A1 - de Kock, Christiaan P. J. A1 - Meyer, Hanno A1 - Dercksen, Vincent J. A1 - Sakmann, Bert T1 - 3D distribution and sub-cellular organization of thalamocortical VPM synapses for individual excitatory neuronal cell types in rat barrel cortex T2 - Conference Abstract No. 173.19/Y35, 39th Annual Meeting of the Society for Neuroscience (SfN) Y1 - 2009 ER -