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  <doc>
    <id>7439</id>
    <completedYear/>
    <publishedYear/>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst/>
    <pageLast/>
    <pageNumber/>
    <edition/>
    <issue/>
    <volume/>
    <type>reportzib</type>
    <publisherName/>
    <publisherPlace/>
    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>0</belongsToBibliography>
    <completedDate>--</completedDate>
    <publishedDate>2019-08-14</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">Serial synapse formation through filopodial competition for synaptic seeding factors</title>
    <abstract language="eng">Following axon pathfinding, growth cones transition from stochastic filopodial exploration to the formation of a limited number of synapses. How the interplay of filopodia and synapse assembly ensures robust connectivity in the brain has remained a challenging problem. Here, we developed a new 4D analysis method for filopodial dynamics and a data-driven computational model of synapse formation for R7 photoreceptor axons in developing Drosophila brains. Our live data support a 'serial synapse formation' model, where at any time point only a single 'synaptogenic' filopodium suppresses the synaptic competence of other filopodia through competition for synaptic seeding factors. Loss of the synaptic seeding factors Syd-1 and Liprin-α leads to a loss of this suppression, filopodial destabilization and reduced synapse formation, which is sufficient to cause the destabilization of entire axon terminals. Our model provides a filopodial 'winner-takes-all' mechanism that ensures the formation of an appropriate number of synapses.</abstract>
    <identifier type="issn">1438-0064</identifier>
    <identifier type="urn">urn:nbn:de:0297-zib-74397</identifier>
    <identifier type="doi">10.1016/j.devcel.2019.06.014</identifier>
    <enrichment key="SourceTitle">Appeared in: Developmental Cell, 50(4), pp. 447-461, 2019</enrichment>
    <author>M. Neset Özel</author>
    <submitter>Josephine Brummer</submitter>
    <author>Abhishek Kulkarni</author>
    <author>Amr Hasan</author>
    <author>Josephine Brummer</author>
    <author>Marian Moldenhauer</author>
    <author>Ilsa-Maria Daumann</author>
    <author>Heike Wolfenberg</author>
    <author>Vincent J. Dercksen</author>
    <author>F. Ridvan Kiral</author>
    <author>Martin Weiser</author>
    <author>Steffen Prohaska</author>
    <author>Max von Kleist</author>
    <author>Peter Robin Hiesinger</author>
    <series>
      <title>ZIB-Report</title>
      <number>19-45</number>
    </series>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>filopodia</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>growth cone dynamics</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>brain wiring</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>2-photon microscopy</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>model</value>
    </subject>
    <collection role="ccs" number="I.">Computing Methodologies</collection>
    <collection role="ccs" number="J.">Computer Applications</collection>
    <collection role="pacs" number="80.00.00">INTERDISCIPLINARY PHYSICS AND RELATED AREAS OF SCIENCE AND TECHNOLOGY</collection>
    <collection role="msc" number="60-XX">PROBABILITY THEORY AND STOCHASTIC PROCESSES (For additional applications, see 11Kxx, 62-XX, 90-XX, 91-XX, 92-XX, 93-XX, 94-XX)</collection>
    <collection role="msc" number="68-XX">COMPUTER SCIENCE (For papers involving machine computations and programs in a specific mathematical area, see Section -04 in that area)</collection>
    <collection role="msc" number="92-XX">BIOLOGY AND OTHER NATURAL SCIENCES</collection>
    <collection role="institutes" number="num">Numerical Mathematics</collection>
    <collection role="institutes" number="vis">Visual Data Analysis</collection>
    <collection role="persons" number="prohaska">Prohaska, Steffen</collection>
    <collection role="persons" number="weiser">Weiser, Martin</collection>
    <collection role="projects" number="FU-Images">FU-Images</collection>
    <collection role="projects" number="GC-Dynamics">GC-Dynamics</collection>
    <collection role="projects" number="BrainWiring">BrainWiring</collection>
    <collection role="institutes" number="VDcC">Visual and Data-centric Computing</collection>
    <file>https://opus4.kobv.de/opus4-zib/files/7439/zibreport-19-45_v1.pdf</file>
  </doc>
</export-example>
