TY - JOUR A1 - Hilbel, Thomas A1 - Feilner, Sven A1 - Struck, Matthias A1 - Hofmann, Christian A1 - Heinig, Andreas A1 - Katus, Hugo A. T1 - Cor/log BAN BT a Wearable Battery Powered mHealth Data Logger and Telemetry Unit for Multiple Vital Sign Monitoring JF - Computing in cardiology N2 - The wireless data logger system “Cor/log® BAN BT” (CL) allows seamless 24/7 monitoring of relevant vital sign parameters. CL covers the entire period of acute point of care inside the hospital and the recovery period, when first mobility is achieved and when the patient is released into an ambulatory or homecare environment. The CL records the relevant vital signs such as ECG, respiration, pulse oximetry with plethysmogram and movement. The vital data collected with the CL data logger is saved on a memory card for further analysis and is simultaneously transmitted in real-time to a telemedicine server via a smartphone or tablet. The smartphone also provides GPS location information. In addition Cor/log View, an Android Application for viewing recorded vital sign data originating from the CL, was developed. CL has also a connector to the generic MedM health cloud. MedM is a generic patient data management system (PDMS) consisting of a cloud portal and a mobile health app. The app runs on Android, iOS and Windows. The app can connects wirelessly to the CL physiologic monitor and stores the vital signs in the cloud. KW - Biomedical monitoring KW - Hospitals KW - Electrocardiography KW - Wireless communication KW - Patient monitoring KW - Wireless sensor networks Y1 - 2016 U6 - https://doi.org/10.22489/CinC.2016.080-163 VL - 43 SP - 273 EP - 276 ER - TY - JOUR A1 - Ettelt, Volker A1 - Belitsky, Alice A1 - Lehnert, Michael A1 - Loidl-Stahlhofen, Angelika A1 - Epple, Matthias A1 - Veith, Michael T1 - Enhanced selective cellular proliferation by multi-biofunctionalisation of medical implant surfaces with heterodimeric BMP-2/6, fibronectin and FGF-2 JF - Journal of biomedical materials research. An official journal of The Society for Biomaterials, The Japanese Society for Biomaterials, and The Australian Society for Biomaterials. A Y1 - 2018 U6 - https://doi.org/10.1002/jbm.a.36480 SN - 1552-4965 VL - 106 IS - 11 SP - 2910 EP - 2922 ER - TY - JOUR A1 - Ettelt, Volker A1 - Ekat, Katharina A1 - Kämmerer, Peer Wolfgang A1 - Kreikemeyer, Bernd A1 - Epple, Matthias A1 - Veith, Michael T1 - Streptavidin-coated surfaces suppress bacterial colonisation by inhibiting non-specific protein adsorption JF - Journal of biomedical materials research : an official journal of The Society for Biomaterials, The Japanese Society for Biomaterials, and The Australian Society for Biomaterials. A N2 - Streptavidin is a 58 kDa tetrameric protein with the highest known affinity to biotin with a wide range of applications in bionanotechnology and molecular biology. Dissolved streptavidin is stable at a broad range of temperature, pH, proteolytic enzymes and exhibits low non‐specific binding. In this study, a streptavidin monolayer was assembled directly on a biotinylated TiO2‐surface to investigate its stability against proteolytic digestion and its suppression of initial bacterial adsorption of Escherichia coli, Bacillus subtilis, and Streptococcus intermedius. In contrast to nonmodified TiO2 surfaces, streptavidin‐coated substrates showed only a negligible non‐specific protein adsorption at physiological protein concentrations as well as a significantly reduced bacterial adhesion. The antiadhesive properties were demonstrated to be the main reason for the suppression of bacterial adhesion, which makes this approach a promising option for future surface biofunctionalization applications. Y1 - 2018 U6 - https://doi.org/10.1002/jbm.a.36276 SN - 1552-4965 VL - 106 IS - 3 SP - 758 EP - 768 PB - New York CY - Wiley ER - TY - JOUR A1 - Brinck, Heinrich A1 - Bührig, C. A1 - Tietz, M. A1 - Krone, Jörg A1 - Siebenborn, T. T1 - Telemikroskopie - komponentenbasiert und hardwareunabhängig JF - Biomedical Engineering / Biomedizinische Technik Y1 - 2002 U6 - https://doi.org/10.1515/bmte.2002.47.s1a.369 SN - 0013-5585 VL - 47 IS - s1a SP - 369 EP - 372 ER - TY - JOUR A1 - Lehnert, Michael A1 - Gorbahn, Miriam A1 - Al-Nawas, Bilal A1 - Köper, Ingo A1 - Knoll, Wolfgang A1 - Veith, Michael T1 - Streptavidin-coated TiO2 surfaces are biologically inert: Protein adsorption and osteoblast adhesion JF - Journal of biomedical materials research. an official journal of The Society for Biomaterials, The Japanese Society for Biomaterials, and The Australian Society for Biomaterials. A N2 - Non‐fouling TiO2 surfaces are attractive for a wide range of applications such as biosensors and medical devices, where biologically inert surfaces are needed. Typically, this is achieved by controlled surface modifications which prevent protein adsorption. For example, polyethylene glycol (PEG) or PEG‐derived polymers have been widely applied to render TiO2 surfaces biologically inert. These surfaces have been further modified in order to achieve specific bio‐activation. Therefore, there have been efforts to specifically functionalize TiO2 surfaces with polymers with embedded biotin motives, which can be used to couple streptavidin for further functionalization. As an alternative, here a streptavidin layer was immobilized by self‐assembly directly on a biotinylated TiO2 surface, thus forming an anti‐adhesive matrix, which can be selectively bio‐activated. The anti‐adhesive properties of these substrates were analyzed by studying the interaction of the surface coating with fibronectin, lysozym, and osteoblast cells using surface plasmon resonance spectroscopy, atomic force microscopy, and light microscopy. In contrast to non‐modified TiO2 surfaces, streptavidin‐coated TiO2 surfaces led to a very biologically inert substrate, making this type of surface coating a promising alternative to polymer coatings of TiO2 surfaces. Y1 - 2012 U6 - https://doi.org/10.1002/jbm.a.33281 SN - 1552-4965 VL - 100A IS - 2 SP - 388 EP - 395 PB - Wiley CY - New York ER - TY - JOUR A1 - Lehnert, Michael A1 - Gorbahn, Miriam A1 - Rosin, Christopher A1 - Klein, Marcus A1 - Köper, Ingo A1 - Al-Nawas, Bilal A1 - Knoll, Wolfgang A1 - Veith, Michael T1 - Adsorption Conformation Behavior of Biotinylated Fibronectin on Streptavidin-Modified TiOx Surfaces Studied by SPR and AFM JF - Langmuir N2 - It is well-known that protein-modified implant surfaces such as TiO2 show a higher bioconductivity. Fibronectin is a glycoprotein from the extracellular matrix (ECM) with a major role in cell adhesion. It can be applied on titanium oxide surfaces to accelerate implant integration. Not only the surface concentration but also the presentation of the protein plays an important role for the cellular response. We were able to show that TiOX surfaces modified with biotinylated fibronectin adsorbed on a streptavidin-silane self-assembly multilayer system are more effective regarding osteoblast adhesion than surfaces modified with nonspecifically bound fibronectin. The adsorption and conformation behavior of biotinylated and nonbiotinylated (native) fibronectin was studied by surface plasmon resonance (SPR) spectroscopy and atomic force microscopy (AFM). Imaging of the protein modification revealed that fibronectin adopts different conformations on nonmodified compared to streptavidin-modified TiOX surfaces. This conformational change of biotinylated fibronectin on the streptavidin monolayer delivers a fibronectin structure similar to the conformation inside the ECM and therefore explains the higher cell affinity for these surfaces. Y1 - 2011 U6 - https://doi.org/10.1021/la200908h SN - 1520-5827 SN - 0743-7463 VL - 27 IS - 12 SP - 7743 EP - 7751 PB - ACS Publishing CY - Washnigton DC ER - TY - JOUR A1 - Lehnert, Michael A1 - Rosin, Christopher A1 - Knoll, Wolfgang A1 - Veith, Michael T1 - Layer-by-Layer Assembly of Streptavidin-Fibronectin-Multilayer on biotinylated TiOx JF - Langmuir N2 - The biomodification of surfaces, especially titanium, is an important issue in current biomedical research. Regarding titanium, it is also important to ensure a specific protein modification of its surface because here protein binding that is too random can be observed. Specific nanoscale architectures can be applied to overcome this problem. As recently shown, streptavidin can be used as a coupling agent to immobilize biotinylated fibronectin (bFn) on a TiOX surface. Because of the conformation of adsorbed biotinylated fibronectin on a streptavidin monolayer, it is possible to adsorb more streptavidin and biotinylated fibronectin layers. On this basis, an alternating protein multilayer can be built up. In contrast to common layer-by-layer technology, in this procedure the mechanism of layer adsorption is very specific because of the interaction of biotin and streptavidin. In addition, we showed that the assembly of this multilayer system and its stability are dependent on the degree of labeling of biotinylated fibronectin. Hence we conclude that it is possible to build up well-defined nanoscale protein architectures by varying the degree of labeling of biotinylated fibronectin. Y1 - 2013 U6 - https://doi.org/10.1021/la303750p VL - 29 IS - 6 SP - 1732 EP - 1737 PB - ACS Publishing CY - Washnigton DC ER - TY - JOUR A1 - Gorbahn, Miriam A1 - Klein, Marcus A1 - Lehnert, Michael A1 - Ziebart, Thomas A1 - Brüllmann, Dan A1 - Köper, Ingo A1 - Wagner, Wilfried A1 - Al-Nawas, Bilal A1 - Veith, Michael T1 - Promotion of Osteogenic Cell Response Using Quasicovalent Immobilized Fibronectin on Titanium Surfaces. Introduction of a Novel Biomimetic Layer System JF - Journal of oral and maxillofacial surgery. official journal of the American Association of Oral and Maxillofacial Surgeons Y1 - 2012 U6 - https://doi.org/10.1016/j.joms.2012.04.004 SN - 0278-2391 SN - 1531-5053 VL - 70 IS - 8 SP - 1827 EP - 1834 PB - Elsevier CY - Maryland Heights, Mo ER - TY - JOUR A1 - Kämmerer, Peer Wolfgang A1 - Lehnert, Michael A1 - Al-Nawas, Bilal A1 - Kumar, Vinay V. A1 - Hagmann, Sebastien A1 - Alshihri, Abdulmonem A1 - Frerich, Bernhard A1 - Veith, Michael T1 - Osseoconductivity of a Specific Streptavidin-Biotin-Fibronectin Surface Coating of Biotinylated Titanium Implants - A Rabbit Animal Study JF - Clinical Implant Dentistry and Related Research Y1 - 2015 U6 - https://doi.org/10.1111/cid.12292 SN - 1708-8208 VL - 17 IS - 2 SP - 601 EP - 612 PB - Wiley CY - Oxford ER - TY - JOUR A1 - Nüßing, Andreas A1 - Wolters, Carsten H. A1 - Brinck, Heinrich A1 - Engwer, Christian T1 - Patient specific simulation of brain stimulation using the unfitted discontinuous galerkin method JF - Brain stimulation. Basic, translational, and clinical research in neuromodulation Y1 - 2015 U6 - https://doi.org/10.1016/j.brs.2015.01.137 SN - 1935-861X VL - 8 IS - 2 SP - 352 ER - TY - JOUR A1 - Nüßing, Andreas A1 - Wolters, Carsten H. A1 - Brinck, Heinrich A1 - Engwer, Christian T1 - The Unfitted Discontinuous Galerkin Method for Solving the EEG Forward Problem JF - IEEE Transactions on Biomedical Engineering Y1 - 2016 U6 - https://doi.org/10.1109/TBME.2016.2590740 VL - 63 IS - 12 SP - 2564 EP - 2575 ER -