TY - CHAP A1 - Gorbahn, Miriam A1 - Lehnert, Michael A1 - Köper, Ingo A1 - Klein, Marcus A1 - Al-Nawas, Bilal A1 - Veith, Michael T1 - Enhancement of Osteogenic Cell Responseon Biomimetic Implant Coatings T2 - Konferenz: 2. Symposium der BiomaTiCs-Gruppe. Universitätsmedizin Mainz, 28. November 2009 in Mainz Y1 - 2009 ER - TY - CHAP A1 - Gorbahn, Miriam A1 - Lehnert, Michael A1 - Klein, Marcus A1 - Brüllmann, Dan A1 - Köper, Ingo A1 - Al-Nawas, Bilal A1 - Veith, Michael T1 - Implant Coating with Fibronectin over a Biotin-Streptavidin system T2 - Konferenz: 3rd International Symposium Interface "Biology of Implants", 15. März 2009 in Rostock (Posterpreis) Y1 - 2009 ER - TY - JOUR A1 - Lehnert, Michael A1 - Gorbahn, Miriam A1 - Rosin, Christopher A1 - Klein, Marcus A1 - Köper, Ingo A1 - Al-Nawas, Bilal A1 - Knoll, Wolfgang A1 - Veith, Michael T1 - Adsorption Conformation Behavior of Biotinylated Fibronectin on Streptavidin-Modified TiOx Surfaces Studied by SPR and AFM JF - Langmuir N2 - It is well-known that protein-modified implant surfaces such as TiO2 show a higher bioconductivity. Fibronectin is a glycoprotein from the extracellular matrix (ECM) with a major role in cell adhesion. It can be applied on titanium oxide surfaces to accelerate implant integration. Not only the surface concentration but also the presentation of the protein plays an important role for the cellular response. We were able to show that TiOX surfaces modified with biotinylated fibronectin adsorbed on a streptavidin-silane self-assembly multilayer system are more effective regarding osteoblast adhesion than surfaces modified with nonspecifically bound fibronectin. The adsorption and conformation behavior of biotinylated and nonbiotinylated (native) fibronectin was studied by surface plasmon resonance (SPR) spectroscopy and atomic force microscopy (AFM). Imaging of the protein modification revealed that fibronectin adopts different conformations on nonmodified compared to streptavidin-modified TiOX surfaces. This conformational change of biotinylated fibronectin on the streptavidin monolayer delivers a fibronectin structure similar to the conformation inside the ECM and therefore explains the higher cell affinity for these surfaces. Y1 - 2011 U6 - https://doi.org/10.1021/la200908h SN - 1520-5827 SN - 0743-7463 VL - 27 IS - 12 SP - 7743 EP - 7751 PB - ACS Publishing CY - Washnigton DC ER - TY - CHAP A1 - Lehnert, Michael A1 - Gorbahn, Miriam A1 - Rosin, Christopher A1 - Klein, Marcus A1 - Köper, Ingo A1 - Al-Nawas, Bilal A1 - Knoll, Wolfgang A1 - Veith, Michael T1 - Streptavidin modified TiOx surfaces for specific bioactivation T2 - Biomaterialien : interdisciplinary journal of functional materials, biomechanics, and tissue engineering Y1 - 2011 UR - https://www.degruyter.com/downloadpdf/j/biomat.2011.12.issue-1-4/biomat.2011.002/biomat.2011.002.pdf SN - 1616-0177 VL - 12 IS - 1-4 SP - 20 EP - 20 PB - deGruyter CY - Berlin; Boston ER - TY - JOUR A1 - Gorbahn, Miriam A1 - Klein, Marcus A1 - Lehnert, Michael A1 - Ziebart, Thomas A1 - Brüllmann, Dan A1 - Köper, Ingo A1 - Wagner, Wilfried A1 - Al-Nawas, Bilal A1 - Veith, Michael T1 - Promotion of Osteogenic Cell Response Using Quasicovalent Immobilized Fibronectin on Titanium Surfaces. Introduction of a Novel Biomimetic Layer System JF - Journal of oral and maxillofacial surgery. official journal of the American Association of Oral and Maxillofacial Surgeons Y1 - 2012 U6 - https://doi.org/10.1016/j.joms.2012.04.004 SN - 0278-2391 SN - 1531-5053 VL - 70 IS - 8 SP - 1827 EP - 1834 PB - Elsevier CY - Maryland Heights, Mo ER - TY - CHAP A1 - Gorbahn, Miriam A1 - Lehnert, Michael A1 - Köper, Ingo A1 - Veith, Michael A1 - Klein, Marcus A1 - Al-Nawas, Bilal T1 - Spezifische Immobilisierung von Fibronektin auf Zahnimplantaten T2 - Konferenz: 1. Symposium der BiomaTiCS-Gruppe: Materialien und Oberflächen in der Chirurgie - Herausforderungen in der Implantologie, 6. Dezember 2008 in Mainz (Posterpreis) Y1 - 2008 ER -