TY - CHAP A1 - Lehnert, Michael A1 - Gorbahn, Miriam A1 - Rosin, Christopher A1 - Köper, Ingo A1 - Knoll, Wolfgang A1 - Veith, Michael T1 - Specific adsorption of Fibronectin on modified titanium Surfaces T2 - Konferenz: 2. Symposium der BiomaTiCs-Gruppe. Universitätsmedizin Mainz, 28. November 2009 in Mainz (Posterpreis) Y1 - 2009 ER - TY - CHAP A1 - Gorbahn, Miriam A1 - Lehnert, Michael A1 - Köper, Ingo A1 - Klein, Marcus A1 - Al-Nawas, Bilal A1 - Veith, Michael T1 - Enhancement of Osteogenic Cell Responseon Biomimetic Implant Coatings T2 - Konferenz: 2. Symposium der BiomaTiCs-Gruppe. Universitätsmedizin Mainz, 28. November 2009 in Mainz Y1 - 2009 ER - TY - CHAP A1 - Gorbahn, Miriam A1 - Lehnert, Michael A1 - Klein, Marcus A1 - Brüllmann, Dan A1 - Köper, Ingo A1 - Al-Nawas, Bilal A1 - Veith, Michael T1 - Implant Coating with Fibronectin over a Biotin-Streptavidin system T2 - Konferenz: 3rd International Symposium Interface "Biology of Implants", 15. März 2009 in Rostock (Posterpreis) Y1 - 2009 ER - TY - JOUR A1 - Lehnert, Michael A1 - Gorbahn, Miriam A1 - Al-Nawas, Bilal A1 - Köper, Ingo A1 - Knoll, Wolfgang A1 - Veith, Michael T1 - Streptavidin-coated TiO2 surfaces are biologically inert: Protein adsorption and osteoblast adhesion JF - Journal of biomedical materials research. an official journal of The Society for Biomaterials, The Japanese Society for Biomaterials, and The Australian Society for Biomaterials. A N2 - Non‐fouling TiO2 surfaces are attractive for a wide range of applications such as biosensors and medical devices, where biologically inert surfaces are needed. Typically, this is achieved by controlled surface modifications which prevent protein adsorption. For example, polyethylene glycol (PEG) or PEG‐derived polymers have been widely applied to render TiO2 surfaces biologically inert. These surfaces have been further modified in order to achieve specific bio‐activation. Therefore, there have been efforts to specifically functionalize TiO2 surfaces with polymers with embedded biotin motives, which can be used to couple streptavidin for further functionalization. As an alternative, here a streptavidin layer was immobilized by self‐assembly directly on a biotinylated TiO2 surface, thus forming an anti‐adhesive matrix, which can be selectively bio‐activated. The anti‐adhesive properties of these substrates were analyzed by studying the interaction of the surface coating with fibronectin, lysozym, and osteoblast cells using surface plasmon resonance spectroscopy, atomic force microscopy, and light microscopy. In contrast to non‐modified TiO2 surfaces, streptavidin‐coated TiO2 surfaces led to a very biologically inert substrate, making this type of surface coating a promising alternative to polymer coatings of TiO2 surfaces. Y1 - 2012 U6 - https://doi.org/10.1002/jbm.a.33281 SN - 1552-4965 VL - 100A IS - 2 SP - 388 EP - 395 PB - Wiley CY - New York ER - TY - JOUR A1 - Lehnert, Michael A1 - Gorbahn, Miriam A1 - Rosin, Christopher A1 - Klein, Marcus A1 - Köper, Ingo A1 - Al-Nawas, Bilal A1 - Knoll, Wolfgang A1 - Veith, Michael T1 - Adsorption Conformation Behavior of Biotinylated Fibronectin on Streptavidin-Modified TiOx Surfaces Studied by SPR and AFM JF - Langmuir N2 - It is well-known that protein-modified implant surfaces such as TiO2 show a higher bioconductivity. Fibronectin is a glycoprotein from the extracellular matrix (ECM) with a major role in cell adhesion. It can be applied on titanium oxide surfaces to accelerate implant integration. Not only the surface concentration but also the presentation of the protein plays an important role for the cellular response. We were able to show that TiOX surfaces modified with biotinylated fibronectin adsorbed on a streptavidin-silane self-assembly multilayer system are more effective regarding osteoblast adhesion than surfaces modified with nonspecifically bound fibronectin. The adsorption and conformation behavior of biotinylated and nonbiotinylated (native) fibronectin was studied by surface plasmon resonance (SPR) spectroscopy and atomic force microscopy (AFM). Imaging of the protein modification revealed that fibronectin adopts different conformations on nonmodified compared to streptavidin-modified TiOX surfaces. This conformational change of biotinylated fibronectin on the streptavidin monolayer delivers a fibronectin structure similar to the conformation inside the ECM and therefore explains the higher cell affinity for these surfaces. Y1 - 2011 U6 - https://doi.org/10.1021/la200908h SN - 1520-5827 SN - 0743-7463 VL - 27 IS - 12 SP - 7743 EP - 7751 PB - ACS Publishing CY - Washnigton DC ER - TY - CHAP A1 - Lehnert, Michael A1 - Gorbahn, Miriam A1 - Rosin, Christopher A1 - Klein, Marcus A1 - Köper, Ingo A1 - Al-Nawas, Bilal A1 - Knoll, Wolfgang A1 - Veith, Michael T1 - Streptavidin modified TiOx surfaces for specific bioactivation T2 - Biomaterialien : interdisciplinary journal of functional materials, biomechanics, and tissue engineering Y1 - 2011 UR - https://www.degruyter.com/downloadpdf/j/biomat.2011.12.issue-1-4/biomat.2011.002/biomat.2011.002.pdf SN - 1616-0177 VL - 12 IS - 1-4 SP - 20 EP - 20 PB - deGruyter CY - Berlin; Boston ER - TY - CHAP A1 - Lehnert, Michael A1 - Gorbahn, Miriam A1 - Rosin, Christopher A1 - Köper, Ingo A1 - Knoll, Wolfgang A1 - Veith, Michael T1 - Biofunctionalization of TiO2 for specific Adsorption of biotinylated Fibronectin T2 - BIOmaterialien Y1 - 2010 SN - 1616-0177 VL - 11 IS - 1 SP - 58 EP - 58 PB - Neuer Merkur GmbH CY - München ER - TY - CHAP A1 - Lehnert, Michael A1 - Gorbahn, Miriam A1 - Rosin, Christopher A1 - Köper, Ingo A1 - Knoll, Wolfgang A1 - Veith, Michael T1 - Specific adsorption of Fibronectin on modified titanium surfaces T2 - Konferenz: Bio meets Nano - Chemical Nanotechnology Talks IX. DECHEMA, 3.-4. Januar 2008 in Frankfurt am Main Y1 - 2008 ER - TY - JOUR A1 - Gorbahn, Miriam A1 - Klein, Marcus A1 - Lehnert, Michael A1 - Ziebart, Thomas A1 - Brüllmann, Dan A1 - Köper, Ingo A1 - Wagner, Wilfried A1 - Al-Nawas, Bilal A1 - Veith, Michael T1 - Promotion of Osteogenic Cell Response Using Quasicovalent Immobilized Fibronectin on Titanium Surfaces. Introduction of a Novel Biomimetic Layer System JF - Journal of oral and maxillofacial surgery. official journal of the American Association of Oral and Maxillofacial Surgeons Y1 - 2012 U6 - https://doi.org/10.1016/j.joms.2012.04.004 SN - 0278-2391 SN - 1531-5053 VL - 70 IS - 8 SP - 1827 EP - 1834 PB - Elsevier CY - Maryland Heights, Mo ER - TY - CHAP A1 - Gorbahn, Miriam A1 - Lehnert, Michael A1 - Köper, Ingo A1 - Veith, Michael A1 - Klein, Marcus A1 - Al-Nawas, Bilal T1 - Spezifische Immobilisierung von Fibronektin auf Zahnimplantaten T2 - Konferenz: 1. Symposium der BiomaTiCS-Gruppe: Materialien und Oberflächen in der Chirurgie - Herausforderungen in der Implantologie, 6. Dezember 2008 in Mainz (Posterpreis) Y1 - 2008 ER - TY - CHAP A1 - Gorbahn, Miriam A1 - Beyer, Andreas A1 - Lehnert, Michael A1 - Köper, Ingo A1 - Veith, Michael T1 - Characterization of the adsorption behaviour of biotinylated Fibronectin on a Streptavidin coated Surface using ELISA technique T2 - Konferenz: Bio meets Nano - Chemical Nanotechnology Talks IX. DECHEMA, 3.-4. Januar 2008 in Frankfurt am Main Y1 - 2008 ER -