TY - JOUR A1 - Uthoff, Jana A1 - Jakobs-Schönwandt, Desiree A1 - Dietz, Karl-Josef A1 - Patel, Anant V. T1 - Development of a Seed Treatment with Pochonia chlamydosporia for Biocontrol Application T2 - Agriculture N2 - Seed treatment is a powerful technique for adding beneficial ingredients to plants during the seed preparation process. Biopolymers as drying agents and delivery systems in seed treatments were investigated for their biocompatibility with blastospores of the nematophagous fungus Pochonia chlamydosporia. To produce a novel seed treatment for the cover crop Phacelia tanacetifolia, xanthan gum TG and gellan gum were the most promising biopolymers in combination with potato starch and bentonite. The seed treatment process as well as the drying process were specially designed to be scalable, which make it suitable for applying the developed seed treatment in agriculture. Application of gellan gum in seed treatments led to 6.3% ± 1.6% of vital blastospores per seed compared to 3.8% ± 0.3% of vital blastospores when applying xanthan gum. Storage tests for seed treatments with 0.5% gellan gum indicated a higher stability at 4 °C compared to storage at 21 °C. After 42 days of storage at 4 °C, 54.1% ± 15.1% of the applied blastospores were viable compared to 0.3% ± 0.8% at 21 °C. This novel seed treatment application with P. chlamydosporia blastospores includes the seed treatment procedure, drying process, and storage tests and can easily be upscaled for application in agriculture. KW - seed treatment KW - Pochonia chlamydosporia KW - biopolymers KW - shelf life Y1 - 2024 UR - https://opus4.kobv.de/opus4-w-hs/frontdoor/index/index/docId/4724 UR - https://nbn-resolving.org/urn:nbn:de:hbz:1010-opus4-47240 UR - https://www.mdpi.com/2077-0472/14/1/138 VL - 2024 IS - 14, Artikel Nr. 138 ER -