Fachbereich Ingenieur- und Naturwissenschaften
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In this paper, different concepts for mixing sample and master mix by means of simulation are discussed. The main focus was on mixers with a low dead volume, because of their relevance to PCR. In addition, a concept for aliquoting the mixed solution was elaborated and simulated. The simulation showed very good mixing with serpentineand tesla-mixers at flow rates of 1-16 µL/s. Furthermore, a uniform distribution with aliquoting could be achieved, while a slight dependence between flow rate and pressure was observed. In the further course, these concepts are to be combined and tested on a microfluidic chip. This can then be integrated into the workflow of existing PCR protocols.
The concept of this thesis is to optimize the manufacturing process of injection moulding tools by using additive manufacturing and thus contribute to environmental protection. For this purpose, the individual steps of the traditional manufacturing process are identified and analysed to determine which steps can be replaced by additive manufacturing. The first step is to clarify which mould components can benefit from additive manufacturing. In particular, the capabilities and limitations of additive manufacturing technologies must be considered.
Furthermore, another focus is on how to optimize the cooling and temperature control of the tools. Potential savings through the use of lightweight or insulating surfaces will be examined. In addition to the technical feasibility, the cost-effectiveness of the concept will also be considered. Finally, the contribution of the concept to the reduction of the environmental impact will be evaluated. In particular, energy savings and the reduction of CO2 emissions are taken into account.
The potential to optimize the injection mould manufacturing process and reduce environmental impact is significant. Using additive manufacturing can be an economic and environmental advantage.
A conductivity-based, batch-optimized system for analyzing the total of carbon parameter in water
(2023)
In cooperation with the company Purification Membranes Analytics GmbH (PMA), a prototype has been developed that analyzes the total organic carbon (TOC) content of water samples based on a complex conductivity measurement. In the process, a batchoptimized system was developed that delivers qualitative results even in highly polluted waters. This method requires small sample volumes and was assembled using an ultrapure water system, a sample mixing system, various electronic control units and a miniTOC system that uses a UV light source to decompose organic carbons and outputs differential values using two conductivity sensors. During the project, a fully automated prototype was developed and is ready for use. In the future, this method could support common methods and lead to more accurate statements on water quality in a cost-effective manner.
Additive manufacturing, also known as 3D printing, has gained tremendous importance in recent years. One of the areas where additive manufacturing is particularly useful is in the fabrication of electrodes. Electrodes are an important component of a wide range of applications, including electrochemistry, biomedical engineering, energy storage, analytics, electronics as well as life sciences. Traditionally, electrodes have been manufactured through costly processes such as etching, electroplating or cutting and milling. Additive manufacturing offers a new way to fabricate electrodes by depositing materials layer by layer (Yap et al., 2015). This opens up new possibilities for designing electrodes with complex geometries and structures that would not be possible using conventional methods. As a result, 3D printed electrodes are gaining interest in fields such as electromobility, water disinfection, manufacturing, and life sciences, which will be presented in this paper.
New technologies leading to better and more efficient light emitting diodes. The emitted radiation reaches higher energy levels and lower wavelengths especially in the UV-C spectrum. UV-C light has a cancer inducing effect on human cells. Therefore it is necessary to work in a safe way with those devices.
This work is focused on an open-source solution for rapid 3D printing of an safe UV-C radiation measuring device. Especially for research groups or universities which can not afford big safety labs.
After modeling and printing the prototype, a series of tests has shown the functionality of that UV-C Diode test system. By using black printing filaments and infill rates over 50 %, the transmission of light could be prevented and the system worked well.
Das Ziel des Projekts ist, den Zustand der Haut von Neurodermitis-Patienten bei verschiedenen Bedingungen und Belastungszuständen erfassen zu können. Dazu wurde ein Prototyp gefertigt, in den ein Mikrosensor zur Bestimmung der Umgebungsfeuchtigkeit, ein Controller mit Bluetooth zur Datenübertragung, sowie ein Lithium-Akku zur Energieversorgung verbaut wurden.
Zur Herstellung von langfristig stabilen Metall-Kautschuk-Verbunden sind chemische Haftvermittler notwendig. Nach Auftrag dieser wird Kautschuk mittels Gummispritzgießen auf die metallischen Grundkörper aufgebracht. In Klein- und Kleinstserien kann der Haftvermittler in präzisen Volumina händisch mit Hilfe eines Fluid-Dispensers aufgetragen werden. Allerdings verhindert dies eine Homogenität und Gleichheit der Schichtdicke, wodurch die Zuverlässigkeit und Stärke der Haftung variieren können. Als technische Lösung wurde ein Pipettierroboter avisiert. Mit Hilfe mehrerer mechanischer und elektrischer Adaptionen – so auch einer neuen Steuerungstechnik – wurden verschiedene Fluid-Dispenser auf 3-Achs-Portalfräsen integriert. Somit konnte die volle Funktionsfähigkeit erreicht werden.
According to the drinking water regulation, drinking water systems must be tested for harmful contamination at specified intervals. To meet the legal requirements and save costs and time, a portable analysis method is to be developed. For this purpose, a concept study was conducted. It describes a portable measuring device for the quantitative detection of legionella using real-time PCR. The study includes the construction elements and conditions that are necessary for the collection, processing, and evaluation of the sample. It was found that commercially available PCR systems do not meet the size requirements and can only be represented by an in-house development. In addition, a microfluidic system must be developed that is designed for one-time use and low production cost.
Das Ziel dieses Projektes war es eine automatisierte Lösung für das Auftragen von Haftvermittlern auf metallische Grundkörper im Nanoliterbereich zu ermöglichen. Für die automatisierte Auftragung von Haftvermittler wurde eine neue Steuerungseinheit auf Grundlage eines Mainboards für die Ansteuerung von 3D-Druckern verwendet, als Firmware diente Marlin. Notwendig waren Anpassungen im Bereich der Motorströme, Geschwindigkeiten, Beschleunigung, Sensitivität, Druckbettgröße und Auswahl von Mainboard und Display. Für die Verfahrwege wurde G-Code mittels eines Programms in Python generiert. Für eine homogene und gleichmäßige Benetzung werden die einzelnen zu benetzenden Objekte angefahren und der Pipettiervorgang gesteuert. Es war möglich die vollständige Funktionsfähigkeit herzustellen.