G-Quadruplexes as An Alternative Recognition Element in Disease-Related Target Sensing

  • G-quadruplexes are made up of guanine-rich RNA and DNA sequences capable of forming noncanonical nucleic acid secondary structures. The base-specific sterical configuration of G-quadruplexes allows the stacked G-tetrads to bind certain planar molecules like hemin (iron (III)-protoporphyrin IX) to regulate enzymatic-like functions such as peroxidase-mimicking activity, hence the use of the term DNAzyme/RNAzyme. This ability has been widely touted as a suitable substitute to conventional enzymatic reporter systems in diagnostics. This review will provide a brief overview of the G-quadruplex architecture as well as the many forms of reporter systems ranging from absorbance to luminescence readouts in various platforms. Furthermore, some challenges and improvements that have been introduced to improve the application of G-quadruplex in diagnostics will be highlighted. As the field of diagnostics has evolved to apply different detection systems, the need for alternative reporter systems such as G-quadruplexes is also paramount.

Download full text files

Export metadata

Additional Services

Search Google Scholar Statistics
Metadaten
Author:Jeunice Ida, Soo Khim Chan, Jörn GlöklerORCiD, Yee Ying Lim, Yee Siew Choong, Theam Soon Lim
URN:urn:nbn:de:kobv:526-opus4-11438
DOI:https://doi.org/10.3390/molecules24061079
ISSN:1420-3049
Parent Title (English):Molecules
Document Type:Article
Language:English
Year of Publication:2019
Publishing Institution:Technische Hochschule Wildau
Release Date:2019/03/21
Tag:DNAzyme; G-quadruplex; colorimetric; diagnostics; fluorescence; luminescence
Volume:24
Issue:6
Source:Ida, J.; Chan, S.K.; Glökler, J.; Lim, Y.Y.; Choong, Y.S.; Lim, T.S. G-Quadruplexes as An Alternative Recognition Element in Disease-Related Target Sensing. Molecules 2019, 24, 1079.
Faculties an central facilities:Fachbereich Ingenieur- und Naturwissenschaften
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 572 Biochemie
Licence (German):Creative Commons - CC BY - Namensnennung 4.0 International
Verstanden ✔
Diese Webseite verwendet technisch erforderliche Session-Cookies. Durch die weitere Nutzung der Webseite stimmen Sie diesem zu. Unsere Datenschutzerklärung finden Sie hier.