Simple paired heavy- and light-chain antibody repertoire sequencing using endoplasmic reticulum microsomes

  • Existing methods for paired antibody heavy- and light-chain repertoire sequencing rely on specialized equipment and are limited by their commercial availability and high costs. Here, we report a novel simple and cost-effective emulsion-based single-cell paired antibody repertoire sequencing method that employs only basic laboratory equipment. We performed a proof-of-concept using mixed mouse hybridoma cells and we also showed that our method can be used for discovery of novel antigen-specific monoclonal antibodies by sequencing human CD19+ B cell IgM and IgG repertoires isolated from peripheral whole blood before and seven days after Td (Tetanus toxoid/Diphtheria toxoid) booster immunization. We anticipate broad applicability of our method for providing insights into adaptive immune responses associated with various diseases, vaccinations, and cancer immunotherapies.

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Metadaten
Author:Praneeth Reddy Devulapally, Jörg Bürger, Thorsten Mielke, Zoltán Konthur, Hans Lehrach, Marie-Laure Yaspo, Jörn GlöklerORCiD, Hans-Jörg Warnatz
URN:urn:nbn:de:kobv:526-opus4-10538
DOI:https://doi.org/10.1186/s13073-018-0542-5
ISSN:1756-994X
Parent Title (English):Genome Medicine
Document Type:Article
Language:English
Year of Publication:2018
Publishing Institution:Technische Hochschule Wildau
Release Date:2018/10/08
Volume:10
First Page:1
Last Page:12
Source:Devulapally, P., Buerger, J., Mielke, T., Konthur, Z., Lehrach, H., Yaspo, M., et al. (2018). Simple paired heavy- and light-chain antibody repertoire sequencing using endoplasmic reticulum microsomes Genome Medicine. 10, 1-12. https://doi.org/10.1186/s13073-018-0542-5
Faculties an central facilities:Fachbereich Ingenieur- und Naturwissenschaften
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie
Licence (German):Creative Commons - CC BY - Namensnennung 4.0 International
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