Engineering of CHO Cells for the Production of Recombinant Glycoprotein Vaccines with Xylosylated N-glycans

  • Xylose is a general component of O-glycans in mammals. Core-xylosylation of N-glycans is only found in plants and helminth. Consequently, xylosylated N-glycans cause immunological response in humans. We have used the F-protein of the human respiratory syncytial virus (RSV), one of the main causes of respiratory tract infection in infants and elderly, as a model protein for vaccination. The RSV-F protein was expressed in CHO-DG44 cells, which were further modified by co-expression of β1,2-xylosyltransferase from Nicotiana tabacum. Xylosylation of RSV-F N-glycans was shown by monosaccharide analysis and MALDI-TOF mass spectrometry. In immunogenic studies with a human artificial lymph node model, the engineered RSV-F protein revealed improved vaccination efficacy.

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Metadaten
Author:Grit Sandig, Hans Henning von Horsten, Lars Radke, Véronique Blanchard, Marcus FrohmeORCiDGND, Christoph Giese, Volker Sandig, Stephan Hinderlich
URN:urn:nbn:de:kobv:526-opus4-9620
DOI:https://doi.org/10.3390/bioengineering4020038
ISSN:2306-5354
Parent Title (German):Bioengineering
Document Type:Article
Language:English
Year of Publication:2017
Publishing Institution:Technische Hochschule Wildau
Release Date:2017/05/15
Tag:CHO; glycoengineering; respiratory syncytial virus; vaccine; xylose
Volume:4
Issue:2
Source:Sandig, G.; von Horsten, H.H.; Radke, L.; Blanchard, V.; Frohme, M.; Giese, C.; Sandig, V.; Hinderlich, S. Engineering of CHO Cells for the Production of Recombinant Glycoprotein Vaccines with Xylosylated N-glycans. Bioengineering 2017, 4, 38.
Faculties an central facilities:Fachbereich Ingenieur- und Naturwissenschaften
Dewey Decimal Classification:6 Technik, Medizin, angewandte Wissenschaften / 66 Chemische Verfahrenstechnik / 660 Chemische Verfahrenstechnik
Licence (German):Creative Commons - CC BY - Namensnennung 4.0 International
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