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Quantitative High-Resolution Genomic Analysis of Single Cancer Cells

  • During cancer progression, specific genomic aberrations arise that can determine the scope of the disease and can be used as predictive or prognostic markers. The detection of specific gene amplifications or deletions in single blood-borne or disseminated tumour cells that may give rise to the development of metastases is of great clinical interest but technically challenging. In this study, we present a method for quantitative high-resolution genomic analysis of single cells. Cells were isolated under permanent microscopic control followed by high-fidelity whole genome amplification and subsequent analyses by fine tiling array-CGH and qPCR. The assay was applied to single breast cancer cells to analyze the chromosomal region centred by the therapeutical relevant EGFR gene. This method allows precise quantitative analysis of copy number variations in single cell diagnostics.

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Metadaten
URN:urn:nbn:de:kobv:526-opus4-6426
Publisher DOI:https://doi.org/10.1371/journal.pone.0026362
Author:Juliane Hannemann, Sönke Meyer-Staeckling, Dirk Kemming, Iris Alpers, Simon A. Joosse, Heike PospisilORCiD, Stefan Kurtz, Jennifer Görndt, Klaus Püschel, Sabine Riethdorf, Klaus Pantel, Burkhard Brandt
ISSN:1932-6203
Parent Title (English):PLoS ONE
Document Type:Article
Language:English
Year of Publication:2011
Volume:6
Issue:11
Page Number:6
Faculties an central facilities:Fachbereich Ingenieurwesen / Wirtschaftsingenieurwesen (bis 8/2014)
Publishing Institution:Technische Hochschule Wildau
Source:Hannemann J, Meyer-Staeckling S, Kemming D, Alpers I, Joosse SA, Pospisil H, et al. (2011) Quantitative High-Resolution Genomic Analysis of Single Cancer Cells. PLoS ONE 6(11): e26362. doi:10.1371/journal.pone.0026362
Dewey Decimal Classification:6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 616 Krankheiten
Licence (German):Creative Commons - CC BY - Namensnennung 2.0 Generic
Release Date:2016/07/06
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