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Re-annotation of the maize oligonucleotide array

  • The microarray technology has become an established approach for large-scale gene expression analysis with mature protocols for sample, microarray, and data processing. The maize oligonucleotide array (maizearray) is one of the few microarray platforms designed for genome-wide gene expression analysis in Zea mays L. Many datas-ets addressing various genetic, physiological and developmental topics generated with this platform are available. The original 57,452 microarray probes were compiled based on expressed sequence tags (ESTs). Meanwhile the maize genome sequence became available providing the possibility for an improved annotation of the microar-ray probe set. In this study we determined the genome positions of all maizearray probes to obtain current gene annotations and generated current Gene Ontology (GO) annotations. These new data allow tracing redundancy of the probe set and interfering cross-hybridizations, and doubled the number of genes with functional GO data. Our re-annotation will largely improve the functional analysis of available and future datasets generated on this microarray platform.

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Metadaten
URN:urn:nbn:de:kobv:526-opus4-6178
URL:https://journals-crea.4science.it/index.php/maydica/article/view/628
Author:Felix Seifert, Alexander Thiemann, Heike PospisilORCiD, Stefan Scholten
ISSN:0025-6153
Parent Title (English):Maydica
Document Type:Article
Language:English
Year of Publication:2012
Volume:57
Issue:1
First Page:49
Last Page:55
Faculties an central facilities:Fachbereich Ingenieurwesen / Wirtschaftsingenieurwesen (bis 8/2014)
Publishing Institution:Technische Hochschule Wildau
Tag:annotation; maize oligonucleotide array; microarray
Source:Seifert et al. Re-annotation of the maize oligonucleotide array. Maydica 57-2012
Dewey Decimal Classification:6 Technik, Medizin, angewandte Wissenschaften / 66 Chemische Verfahrenstechnik / 660 Chemische Verfahrenstechnik
Licence (German):Das Dokument ist urheberrechtlich geschützt.
Release Date:2016/06/30
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