The Aquaporin Channel Repertoire of the Tardigrade Milnesium tardigradum

  • Limno-terrestrial tardigrades are small invertebrates that are subjected to periodic drought of their micro-environment. They have evolved to cope with these unfavorable conditions by anhydrobiosis, an ametabolic state of low cellular water. During drying and rehydration, tardigrades go through drastic changes in cellular water content. By our transcriptome sequencing effort of the limno-terrestrial tardigrade Milnesium tardigradum and by a combination of cloning and targeted sequence assembly, we identified transcripts encoding eleven putative aquaporins. Analysis of these sequences proposed 2 classical aquaporins, 8 aquaglyceroporins and a single potentially intracellular unorthodox aquaporin. Using quantitative real-time PCR we analyzed aquaporin transcript expression in the anhydrobiotic context. We have identified additional unorthodox aquaporins in various insect genomes and have identified a novel common conserved structural feature in these proteins. Analysis of the genomic organization of insect aquaporin genes revealed several conserved gene clusters.

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Metadaten
Author:Markus GrohmeGND, Brahim Mali, Weronika Wełnicz, Stephanie Michel, Ralph O. Schill, Marcus FrohmeORCiDGND
URN:urn:nbn:de:kobv:526-opus4-6092
DOI:https://doi.org/10.4137/BBI.S11497
ISSN:1177-9322
Parent Title (English):Bioinformatics and Biology Insights
Document Type:Article
Language:English
Year of Publication:2013
Year of first Publication:2013
Publishing Institution:Technische Hochschule Wildau
Release Date:2016/06/30
Tag:anhydrobiosis; tardigrade; unorthodox aquaporin
Volume:2013
Issue:7
First Page:153
Last Page:165
Source:Grohme et al. The Aquaporin Channel Repertoire of the Tardigrade Milnesium tardigradum. Bioinformatics and Biology Insights 2013:7 153-165. DOI: 10.4137/BBI.S11497
Faculties an central facilities:Fachbereich Ingenieurwesen / Wirtschaftsingenieurwesen (bis 8/2014)
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie
Licence (German):Creative Commons - CC BY-NC 3.0 - Namensnennung-Nicht kommerziell 3.0 Unported
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