Überblick Statistik: Seitenaufrufe (grau) und PDF-Downloads (blau)

Filtering duplicate reads from 454 pyrosequencing data

  • Throughout the recent years, 454 pyrosequencing has emerged as an efficient alternative to traditional Sanger sequencing and is widely used in both de novo whole-genome sequencing and metagenomics. Especially the latter application is extremely sensitive to sequencing errors and artificially duplicated reads. Both are common in 454 pyrosequencing and can create a strong bias in the estimation of diversity and composition of a sample. To date, there are several tools that aim to remove both sequencing noise and duplicates. Nevertheless, duplicate removal is often based on nucleotide sequences rather than on the underlying flow values, which contain additional information.

Download full text files

Export metadata

Additional Services

Search Google Scholar
Metadaten
URN:urn:nbn:de:kobv:526-opus4-6080
Publisher DOI:https://doi.org/10.1093/bioinformatics/btt047
Author:Susanne Balzer, Ketil Malde, Markus GrohmeORCiDGND, Inge Jonassen
ISSN:1367-4811
Parent Title (English):Bioinformatics
Document Type:Article
Language:English
Year of Publication:2013
Volume:29
Issue:7
First Page:830
Last Page:836
Faculties an central facilities:Fachbereich Ingenieurwesen / Wirtschaftsingenieurwesen (bis 8/2014)
Date of first Publication:2013/02/01
Publishing Institution:Technische Hochschule Wildau
Source:S.Balzer et al. Filtering duplicate reads from 454 pyrosequencing data. Vol. 29 no. 7 2013, pages 830–836. doi:10.1093/bioinformatics/btt047
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie
Licence (German):Creative Commons - CC BY 3.0 - Namensnennung 3.0 Unported
Release Date:2016/06/30
Verstanden ✔
Diese Webseite verwendet technisch erforderliche Session-Cookies. Durch die weitere Nutzung der Webseite stimmen Sie diesem zu. Unsere Datenschutzerklärung finden Sie hier.