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Screening and genetic characterization of thermo-tolerant Synechocystis sp. PCC6803 strains created by adaptive evolution

  • Temperature tolerance is an important aspect for commercial scale outdoor cultivation of microalgae and cyanobacteria. While various genes are known to be related to Synechocystis sp. PCC6803's heat shock response, there is very limited published data concerning the specific genes involved in long term thermal tolerance. We have previously used random mutagenesis and adaptive evolution to generate a mixture of strains of Synechocystis sp. PCC6803 with significantly increased thermal tolerance. The genetic modifications leading to the phenotypes of the newly generated strains are the focus of this work.

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Metadaten
URN:urn:nbn:de:kobv:526-opus4-6014
Publisher DOI:https://doi.org/10.1186/1472-6750-14-66
Author:Ulrich M. TillichGND, Nick Wolter, Philipp Franke, Ulf Dühring, Marcus FrohmeORCiDGND
ISSN:1472-6750
Parent Title (English):BMC Biotechnology
Document Type:Article
Language:English
Year of Publication:2014
Volume:14
Issue:66
Page Number:15
Faculties an central facilities:Fachbereich Ingenieurwesen / Wirtschaftsingenieurwesen (bis 8/2014)
Date of first Publication:2014/07/17
Publishing Institution:Technische Hochschule Wildau
Tag:HTS; NGS; adaptive evolution; cyanobacteria; synechocystis; thermal tolerance
Source:Tillich et al.:Screening and genetic characterizationof thermo-tolerant Synechocystis sp. PCC6803 strains created byadaptive evolution. BMC Biotechnology 2014 14:66. doi:10.1186/1472-6750-14-66
Dewey Decimal Classification:6 Technik, Medizin, angewandte Wissenschaften / 66 Chemische Verfahrenstechnik / 660 Chemische Verfahrenstechnik
Licence (German):Creative Commons - CC BY - Namensnennung 4.0 International
Release Date:2016/06/29
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