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Intact cell mass spectrometry as a rapid and specific tool for the differentiation of toxic effects in cell-based ecotoxicological test systems

  • In the last few decades, MALDI-TOF MS has become a useful technique not only in proteomics, but also as a fast and specific tool for whole cell analysis through intact cell mass spectrometry (IC-MS). The present study evaluated IC-MS as a novel tool for the detection of distinct patterns that can be observed after exposure to a certain toxin or concentration by utilizing the eukaryotic fish cell line RTL-W1. Two different viability assays were performed to define the range for IC-MS investigations, each of which employing copper sulfate, acridine, and β-naphthoflavone (BNF) as model compounds for several classes of environmental toxins. The IC-MS of RTL-W1 cells revealed not only specific spectral patterns for the various toxins, but also that the concentration used had an effect on RTL-W1 profiles. After the exposure with copper sulfate and acridine, the spectra of RTL-W1 showed a significant increase of certain peaks in the higher mass range (m/z >7000), which is probably attributed to the apoptosis of RTL-W1. On the contrary, exposure to BNF showed a distinct change of ion abundances only in the lower mass range (m/z <7000). Furthermore, a set of mass peaks could be identified as a specific biomarker for a single toxin treatment, so IC-MS demonstrates a new method for the distinction of toxic effects in fish cells. Due to fast sample preparation and high throughput, IC-MS offers great potential for ecotoxicological studies to investigate cellular effects of different substances and complex environmental samples.

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Metadaten
URN:urn:nbn:de:kobv:526-opus4-5900
Publisher DOI:https://doi.org/10.1007/s00216-015-8937-2
Author:Liane Kober, Henriette Meyer-Alert, Desirée Grienitz, Henner HollertORCiD, Marcus FrohmeORCiDGND
ISSN:1618-2650
Parent Title (English):Analytical and Bioanalytical Chemistry
Document Type:Article
Language:English
Year of Publication:2015
Volume:407
Issue:25
First Page:7721
Last Page:7731
Faculties an central facilities:Fachbereich Ingenieur- und Naturwissenschaften
Year of first Publication:2015
Publishing Institution:Technische Hochschule Wildau
Tag:MALDI; RTL-W1; acridine; copper sulfate; intact cell mass spectrometry; β-Naphthoflavone
Source:Sascha Liane Kober, Henriette Meyer-Alert, Desirée Grienitz, Henner Hollert, Marcus Frohme. Intact cell mass spectrometry as a rapid and specific tool for the differentiation of toxic effects in cell-based ecotoxicological test systems. Anal Bioanal Chem (2015) 407:7721–7731. DOI 10.1007/s00216-015-8937-2
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 54 Chemie / 543 Analytische Chemie
Licence (German):Creative Commons - CC BY - Namensnennung 4.0 International
Release Date:2016/06/28
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