Towards a novel bioelectrocatalytic platform based on “wiring” of pyrroloquinoline quinone-dependent glucose dehydrogenase with an electrospun conductive polymeric fiber architecture

  • Electrospinning is known as a fabrication technique for electrode architectures that serve as immobilization matrices for biomolecules. The current work demonstrates a novel approach to construct a conductive polymeric platform, capable not only of immobilization, but also of electrical connection of the biomolecule with the electrode. It is produced upon electrospinning from mixtures of three different highly conductive sulfonated polyanilines and polyacrylonitrile on ITO electrodes. The resulting fiber mats are with a well-retained conductivity. After coupling the enzyme pyrroloquinoline quinone-dependent glucose dehydrogenase (PQQ-GDH) to polymeric structures and addition of the substrate glucose an efficient bioelectrocatalysis is demonstrated. Depending on the choice of the sulfonated polyanilline mediatorless bioelectrocatalysis starts at low potentials; no large overpotential is needed to drive the reaction. Thus, the electrospun conductive immobilization matrix acts here as a transducing element, representing a promising strategy to use 3D polymeric scaffolds as wiring agents for active enzymes. In addition, the mild and well reproducible fabrication process and the active role of the polymer film in withdrawing electrons from the reduced PQQ-GDH lead to a system with high stability. This could provide access to a larger group of enzymes for bioelectrochemical applications including biosensors and biofuel cells.

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Metadaten
Author:Johannes Gladisch, David Sarauli, Daniel Schäfer, Birgit Dietzel, Burkhard Schulz, Fred LisdatORCiDGND
URN:urn:nbn:de:kobv:526-opus4-5846
DOI:https://doi.org/10.1038/srep19858
ISSN:2045-2322
Parent Title (English):Scientific Reports
Document Type:Article
Language:English
Year of Publication:2016
Date of first Publication:2016/01/29
Publishing Institution:Technische Hochschule Wildau
Release Date:2016/06/16
Volume:6
Page Number:10
Source:Gladisch, J. et al. Towards a novel bioelectrocatalytic platform based on “wiring’’ of pyrroloquinoline quinone-dependent glucose dehydrogenase with an electrospun conductive polymeric fiber architecture. Sci. Rep.6, 19858; doi: 10.1038/srep19858 (2016).
Faculties an central facilities:Fachbereich Ingenieur- und Naturwissenschaften
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie
Licence (German):Creative Commons - CC BY - Namensnennung 4.0 International
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