Carboxylated or Aminated Polyaniline—Multiwalled Carbon Nanotubes Nanohybrids for Immobilization of Cellobiose Dehydrogenase on Gold Electrodes
- Polymer-multiwalled carbon nanotube (MWCNT) nanohybrids, which differ in surface charge have been synthesized to study the bioelectrocatalysis of adsorbed cellobiose dehydrogenase (CDH) from Phanerochaete sordida on gold electrodes. To obtain negatively charged nanohybrids, poly(3-amino-4-methoxybenzoic acid-co-aniline) (P(AMB-A)) was covalently linked to the surface of MWCNTs while modification with p-phenylenediamine (PDA) converted the COOH-groups to positively charged amino groups. Fourier transform infrared spectroscopy (FTIR) measurements verified the p-phenylenediamine (PDA) modification of the polymer-CNT nanohybrids. The positively charged nanohybrid MWCNT-P(AMB-A)-PDA promoted direct electron transfer (DET) of CDH to the electrode and bioelectrocatalysis of lactose was observed. Amperometric measurements gave an electrochemical response with KMapp = 8.89 mM and a current density of 410 nA/cm2 (15 mM lactose). The catalytic response was tested at pH 3.5 and 4.5. Interference by ascorbic acid was not observed. The study proves that DET between the MWCNT-P(AMB-A)-PDA nanohybrids and CDH is efficient and allows the sensorial detection of lactose.
| URN: | urn:nbn:de:kobv:526-opus4-5826 |
|---|---|
| Publisher DOI: | https://doi.org/10.3390/bios4040370 |
| Author: | Johannes TanneGND, Daniel Kracher, Birgit DietzelORCiD, Burkhard Schulz, Roland Ludwig, Fred LisdatORCiDGND, Frieder W. SchellerORCiD, Frank Fabian BierORCiD |
| ISSN: | 2079-6374 |
| Parent Title (English): | Biosensors |
| Document Type: | Article |
| Language: | English |
| Year of Publication: | 2014 |
| Volume: | 4 |
| Issue: | 4 |
| First Page: | 370 |
| Last Page: | 386 |
| Date of first Publication: | 2014/10/22 |
| Publishing Institution: | Technische Hochschule Wildau |
| Tag: | cellobiose dehydrogenase; multiwalled carbon nanotube; nanohybrids; polyaniline |
| Source: | Tanne, J.; Kracher, D.; Dietzel, B.; Schulz, B.; Ludwig, R.; Lisdat, F.; Scheller, F.W.; Bier, F.F. Carboxylated or Aminated Polyaniline—Multiwalled Carbon Nanotubes Nanohybrids for Immobilization of Cellobiose Dehydrogenase on Gold Electrodes. Biosensors 2014, 4, 370-386. |
| Faculties an central facilities: | Fachbereich Ingenieur- und Naturwissenschaften |
| Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
| Licence (German): | Creative Commons - CC BY - Namensnennung 4.0 International |
| Release Date: | 2016/06/16 |


