Carboxylated or Aminated Polyaniline—Multiwalled Carbon Nanotubes Nanohybrids for Immobilization of Cellobiose Dehydrogenase on Gold Electrodes

  • Polymer-multiwalled carbon nanotube (MWCNT) nanohybrids, which differ in surface charge have been synthesized to study the bioelectrocatalysis of adsorbed cellobiose dehydrogenase (CDH) from Phanerochaete sordida on gold electrodes. To obtain negatively charged nanohybrids, poly(3-amino-4-methoxybenzoic acid-co-aniline) (P(AMB-A)) was covalently linked to the surface of MWCNTs while modification with p-phenylenediamine (PDA) converted the COOH-groups to positively charged amino groups. Fourier transform infrared spectroscopy (FTIR) measurements verified the p-phenylenediamine (PDA) modification of the polymer-CNT nanohybrids. The positively charged nanohybrid MWCNT-P(AMB-A)-PDA promoted direct electron transfer (DET) of CDH to the electrode and bioelectrocatalysis of lactose was observed. Amperometric measurements gave an electrochemical response with KMapp = 8.89 mM and a current density of 410 nA/cm2 (15 mM lactose). The catalytic response was tested at pH 3.5 and 4.5. Interference by ascorbic acid was not observed. The study proves that DET between the MWCNT-P(AMB-A)-PDA nanohybrids and CDH is efficient and allows the sensorial detection of lactose.

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Metadaten
Author:Johannes Tanne, Daniel Kracher, Birgit Dietzel, Burkhard Schulz, Roland Ludwig, Fred LisdatORCiDGND, Frieder W. Scheller, Frank Fabian Bier
URN:urn:nbn:de:kobv:526-opus4-5826
DOI (Version of Record):https://doi.org/10.3390/bios4040370
ISSN:2079-6374
Parent Title (English):Biosensors
Document Type:Article
Language:English
Year of Publication:2014
Date of first Publication:2014/10/22
Publishing Institution:Technische Hochschule Wildau
Contributing Corporation:Fraunhofer Institute for Cell Therapy and Immunology (IZI-BB), Branch Bioanalytics and Bioprocesses Potsdam-Golm; Food Biotechnology Laboratory, Department of Food Sciences and Technology, BOKU-University of Natural Resources and Life Sciences; Institute for Thin Film and Microsensoric Technology
Release Date:2016/06/16
Tag:cellobiose dehydrogenase; multiwalled carbon nanotubes; nanohybrids; polyaniline
Volume:4
Issue:4
First Page:370
Last Page:386
Source:Tanne, J.; Kracher, D.; Dietzel, B.; Schulz, B.; Ludwig, R.; Lisdat, F.; Scheller, F.W.; Bier, F.F. Carboxylated or Aminated Polyaniline—Multiwalled Carbon Nanotubes Nanohybrids for Immobilization of Cellobiose Dehydrogenase on Gold Electrodes. Biosensors 2014, 4, 370-386.
Faculties an central facilities:Fachbereich Ingenieur- und Naturwissenschaften
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie
Licence (German):Creative Commons - CC BY - Namensnennung 4.0 International
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