Überblick Statistik: Seitenaufrufe (grau) und PDF-Downloads (blau)

Light triggered detection of aminophenyl phosphate with a quantum dot based enzyme electrode

  • An electrochemical sensor for p-aminophenyl phosphate (p APP) is reported. It is based on the electrochemical conversion of 4-aminophenol (4AP) at a quantum dot (QD) modified electrode under illumination. Without illumination no electron transfer and thus no oxidation of 4AP can occur. p APP as substrate is converted by the enzyme alkaline phosphatase (ALP) to generate 4AP as a product. The QDs are coupled via 1,4-benzenedithiol (BDT) linkage to the surface of a gold electrode and thus allow potential-controlled photocurrent generation. The photocurrent is modified by the enzyme reaction providing access to the substrate detection. In order to develop a photobioelectrochemical sensor the enzyme is immobilized on top of the photo-switchable layer of the QDs. Immobilization of ALP is required for the potential possibility of spatially resolved measurements. Geometries with immobilized ALP are compared versus having the ALP in solution. Data indicate that functional immobilization with layer-by-layer assembly is possible. Enzymatic activity of ALP and thus the photocurrent can be described by Michaelis- Menten kinetics. p APP is detected as proof of principle investigation within the range of 25 μM - 1 mM.

Download full text files

Export metadata

Additional Services

Search Google Scholar
Metadaten
URN:urn:nbn:de:kobv:526-opus4-5808
Publisher DOI:https://doi.org/10.1186/1477-3155-9-46
Author:Waqas Khalid, Gero GöbelORCiD, Dominik Hühn, Jose-Maria Montenegro, Pilar Rivera-Gil, Fred LisdatORCiDGND, Wolfgang J. ParakORCiD
ISSN:1477-3155
Parent Title (English):Journal of Nanobiotechnology
Document Type:Article
Language:English
Year of Publication:2011
Volume:9
Issue:46
Page Number:10
Faculties an central facilities:Fachbereich Ingenieurwesen / Wirtschaftsingenieurwesen (bis 8/2014)
Date of first Publication:2011/10/07
Publishing Institution:Technische Hochschule Wildau
Source:Khalid, W., Göbel, G., Hühn, D. et al. Light triggered detection of aminophenyl phosphate with a quantum dot based enzyme electrode. J Nanobiotechnol 9, 46 (2011). https://doi.org/10.1186/1477-3155-9-46
Dewey Decimal Classification:6 Technik, Medizin, angewandte Wissenschaften / 66 Chemische Verfahrenstechnik / 660 Chemische Verfahrenstechnik
Licence (German):Creative Commons - CC BY - Namensnennung 2.0 Generic
Release Date:2016/06/16
Verstanden ✔
Diese Webseite verwendet technisch erforderliche Session-Cookies. Durch die weitere Nutzung der Webseite stimmen Sie diesem zu. Unsere Datenschutzerklärung finden Sie hier.